Possible regulation of trehalose metabolism by methylation in Saccharomyces cerevisiae

Journal of Cellular Physiology
Shinjinee SenguptaAnil K Ghosh

Abstract

The current study was undertaken to correlate post-translational protein modification by methylation with the functionality of enzymes involved in trehalose metabolism in Saccharomyces cerevisiae. Trehalose is an economically important disaccharide providing protection against various kinds of stresses. It also acts as a source of cellular energy by storing glucose. Methyl group donor S-adenosyl L-methionine (AdoMet) and methylation inhibitor-oxidized adenosine (AdOx) were used for the methylation study. AdoMet delayed initial growth of the cells but the overall growth rate remained same suggesting its interference in G1 phase of the cell cycle. Metabolic-altered enzyme activities of acid trehalase (AT), neutral trehalase (NT), and trehalose-6-phosphate synthase (TPS) were observed when treated with AdOx and AdoMet separately. A positive effect of methylation was observed in TPS, hence, it was purified in three different conditions, using AdoMet, AdOx, and control. Differences in mobility of methylated, methylation-inhibited, and control TPS during acidic native gel electrophoresis confirmed the occurrence of induced methylation. Hydrolysis under alkaline pH conditions revealed that methylation of TPS was different than O-methy...Continue Reading

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Citations

Apr 27, 2016·Chemical Communications : Chem Comm·Yixin ZhangZongbao K Zhao
Oct 6, 2018·Biochimica Et Biophysica Acta. Molecular Cell Research·Farida TripodiPaola Coccetti

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