Possible role of prostaglandins in the regulation of mouse myoblasts

Journal of Cellular Physiology
M J RossiS M Steiner

Abstract

A differentiation-defective mouse myoblast subclone (DD-1), cells of which do not fuse into myotubes nor synthesize muscle-specific proteins, was employed to help define the role of eicosanoids in mouse myoblast differentiation. We observed by hplc, tlc, and radioimmunoassay that the DD-1 cells release strikingly higher levels of cyclooxygenase pathway products prostaglandin E2 and F2 alpha into the culture medium than the parental non-differentiation-defective cells (DZ). In contrast, the levels of 15-hydroxyeicosatetraenoic acid (15-HETE), a lipoxygenase product, and a putatively identified second lipoxygenase product (LLP) did not differ greatly in the two cell types. The DD-1 cells also have strikingly higher levels of cyclooxygenase activity than the parental cells as determined by intact and broken cell assays. Additional fusion-defective clones were isolated on the basis of their flattened appearance and ability to grow in "mitogen-poor" medium and these cells also released strikingly higher levels of prostaglandins E2 and F2 alpha into the growth medium. The "turn on" of the cyclooxygenase pathway in the DD-1 cells and other fusion-defective cells is consistent with the hypothesis that the products of this pathway contr...Continue Reading

References

Sep 1, 1977·Developmental Biology·R J Zalin
Apr 22, 1987·Biochimica Et Biophysica Acta·M D Mitchell
Jun 1, 1988·Journal of Cellular Physiology·G G SkouterisL A Stocken
Dec 1, 1973·Journal of Bacteriology·S SteinerR L Lester
Sep 1, 1983·Journal of Cellular Physiology·E RozengurtM Keehan
Nov 1, 1984·Experimental Cell Research·S SteinerT Adams
Jan 1, 1980·Journal of Supramolecular Structure·T A LinkhartS D Hauschka
Jun 11, 1982·Biochimica Et Biophysica Acta·T Ruzicka, M P Printz

❮ Previous
Next ❯

Citations

Jun 16, 2009·Journal of Muscle Research and Cell Motility·M MondaB De Luca
Apr 16, 2003·The Journal of Cell Biology·Valerie Horsley, Grace K Pavlath
Jan 1, 1991·Pathology, Research and Practice·M D Grounds
Sep 20, 2005·Experimental Cell Research·Jeffrey S OtisGrace K Pavlath
Jul 12, 2011·Neurobiology of Disease·Daniel BeaulieuJack Puymirat
Apr 16, 2004·American Journal of Physiology. Cell Physiology·Brenda A BondesenGrace K Pavlath
Feb 10, 2006·American Journal of Physiology. Cell Physiology·Brenda A BondesenGrace K Pavlath
Dec 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kinga K TomczakAlan H Beggs
May 10, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brenda A BondesenGrace K Pavlath

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.