Possible role of the Nipah virus V protein in the regulation of the interferon beta induction by interacting with UBX domain-containing protein1

Scientific Reports
Shotaro UchidaMisako Yoneda

Abstract

Nipah virus (NiV) is a highly pathogenic paramyxovirus that causes lethal encephalitis in humans. We previously reported that the V protein, one of the three accessory proteins encoded by the P gene, is one of the key determinants of the pathogenesis of NiV in a hamster infection model. Satterfield B.A. et al. have also revealed that V protein is required for the pathogenicity of henipavirus in a ferret infection model. However, the complete functions of NiV V have not been clarified. In this study, we identified UBX domain-containing protein 1 (UBXN1), a negative regulator of RIG-I-like receptor signaling, as a host protein that interacts with NiV V. NiV V interacted with the UBX domain of UBXN1 via its proximal zinc-finger motif in the C-terminal domain. NiV V increased the level of UBXN1 protein by suppressing its proteolysis. Furthermore, NiV V suppressed RIG-I and MDA5-dependent interferon signaling by stabilizing UBXN1 and increasing the interaction between MAVS and UBXN1 in addition to directly interrupting the activation of MDA5. Our results suggest a novel molecular mechanism by which the induction of interferon is potentially suppressed by NiV V protein via UBXN1.

References

May 29, 2000·Science·K B ChuaB W Mahy
Mar 13, 2001·Journal of Molecular Biology·A BuchbergerM Bycroft
Sep 15, 2004·The Biochemical Journal·Helen McNeillPhilip Cohen
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·J AndrejevaR E Randall
Jan 25, 2005·Emerging Infectious Diseases·Vincent P HsuRobert F Breiman
Jun 10, 2005·Journal of Biochemistry·Takamasa IshibashiHiroyuki Sugiyama
Dec 17, 2005·Nature Reviews. Microbiology·Bryan T EatonLin-Fa Wang
Feb 24, 2006·Emerging Infectious Diseases·Mandeep S ChadhaAkhilesh Mishra
Oct 20, 2006·Virology·Kay ChildsStephen Goodbourn
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Misako YonedaChieko Kai
Mar 21, 2007·Emerging Infectious Diseases·Catherine IehléJean-Marc Reynes
Apr 19, 2008·The Journal of General Virology·Katrina SleemanPaul A Rota
Apr 29, 2008·Cellular and Molecular Life Sciences : CMLS·C Schuberth, A Buchberger
May 1, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M Jahangir HossainRobert F Breiman
Jul 24, 2008·PloS One·David T S HaymanAndrew A Cunningham
Nov 8, 2008·Nature Reviews. Immunology·Andrew G Bowie, Leonie Unterholzner
Dec 3, 2008·Emerging Infectious Diseases·Yan LiZhengli Shi
Dec 8, 2010·The Journal of Biological Chemistry·David P LaLonde, Anthony Bretscher
May 3, 2011·Emerging Infectious Diseases·Vidya A ArankalleAkhilesh C Mishra
Apr 26, 2012·Journal of Virology·Takashi IrieTakemasa Sakaguchi
Aug 11, 2012·PLoS Pathogens·Glenn A MarshLin-Fa Wang
Oct 24, 2012·The Journal of Infectious Diseases·Kévin P DhondtBranka Horvat
Jan 5, 2013·Science·Le CongFeng Zhang
Nov 19, 2014·Nature Communications·Olivier PernetBenhur Lee
Jan 28, 2015·Emerging Infectious Diseases·Paola Katrina G ChingA Ruth Foxwell
Jun 25, 2015·Nature Communications·Benjamin A SatterfieldChad E Mire

❮ Previous
Next ❯

Citations

Apr 28, 2020·Combinatorial Chemistry & High Throughput Screening·Praveen K P KrishnamoorthyPrasanna D Revanasiddappa
Jun 3, 2021·Antiviral Research·Moushimi AmayaChristopher C Broder
Aug 6, 2021·Antioxidants & Redox Signaling·Arjun SharmaMarkus Bosmann

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation assay
confocal microscopy
immunoprecipitation
pull-down
transfection
PCR
electrophoresis
Assay

Software Mentioned

Mascot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.