Possible treatment for cutaneous lichen planus: An in vitro anti-inflammatory role of Angelica polysaccharide in human keratinocytes HaCaT.

International Journal of Immunopathology and Pharmacology
Jun WangChengfei Guan

Abstract

Cutaneous lichen planus (CLP) is an autoimmune disease. Angelica polysaccharide (AP) has been found to exert immunomodulation activity. In this study, we explored the roles of AP in lipopolysaccharide (LPS)-induced inflammatory injury of human keratinocytes (HaCaT cells), as well as the underlying mechanisms. LPS-induced cell injury was evaluated by alterations of cell viability, apoptosis, and expressions of proteins associated with apoptosis and inflammatory cytokines. Then, the protective effects of AP on LPS-induced cell injury were assessed. The protein expressions of sirtuin 1 (SIRT1) and key kinases in the Nrf2/HO-1 and nuclear factor κB (NF-κB) pathways were measured using western blotting. SIRT1 knockdown and overexpression were used to analyze whether AP affected HaCaT cells through regulating SIRT1. Finally, the possible inhibitory effects of AP on cell injury after LPS treatment were also evaluated. We found that LPS reduced HaCaT cell viability, enhanced apoptosis, and induced release of inflammatory cytokines. AP alleviated LPS-induced HaCaT cell inflammatory injury. The expression of SIRT1 was enhanced after AP treatment. AP activated Nrf2/HO-1 pathway while inhibited NF-κB pathway in HaCaT cells. The protective ...Continue Reading

References

Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Sep 20, 2001·Genes & Development·N Silverman, T Maniatis
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 23, 2010·Aging Cell·Gizem Donmez, Leonard Guarente
Dec 15, 2010·Anais Brasileiros De Dermatologia·Alan Motta do CantoPaulo Sérgio da Silva Santos
Nov 1, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hong-Feng YuanXue-Tao Pei
Feb 24, 2012·The New England Journal of Medicine·Laurence Le Cleach, Olivier Chosidow
Dec 18, 2013·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Rui LuGang Zhou
Jun 4, 2014·Indian Journal of Dermatology·Sandeep K AroraRanzana W Minz
Dec 30, 2014·Journal of the European Academy of Dermatology and Venereology : JEADV·P-I ChungH-N Liu
Jun 2, 2015·The International Journal of Biochemistry & Cell Biology·Danilo RanieriMaria Rosaria Torrisi
Jun 30, 2015·Indian Journal of Dermatology·Sonia Gupta, Manveen Kaur Jawanda
Jan 8, 2016·International Immunopharmacology·Kaiping WangYu Zhang
Feb 20, 2016·International Journal of Biological Macromolecules·Chao ZhuangHong-Guang Xie
May 21, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Wei-An MaoYing Ye
Sep 10, 2017·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wen-Feng ZhangMing-Hua Duan

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Citations

Aug 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuzhu HeOk-Joon Kim
Feb 3, 2021·Antioxidants·Peter F SuraiMichael T Kidd
Mar 23, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Tomás G VillaCarmen Sieiro
May 1, 2021·Cells·Angelika Bielach-BazylukTomasz Hryszko
Jun 7, 2021·International Journal of Biological Macromolecules·Jijuan NaiHuagang Sheng

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Methods Mentioned

BETA
flow cytometry
FACS
Protein Assay
electrophoresis
transfection

Software Mentioned

ImageJ
FlowJo
Graphpad Prism

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