Post-crossing segment of dI1 commissural axons forms collateral branches to motor neurons in the developing spinal cord

The Journal of Comparative Neurology
Takeshi Kaneyama, R Shirasaki

Abstract

The dI1 commissural axons in the developing spinal cord, upon crossing the midline through the floor plate, make a sharp turn to grow rostrally. These post-crossing axons initially just extend adjacent to the floor plate without entering nearby motor columns. However, it remains poorly characterized how these post-crossing dI1 axons behave subsequently to this process. In the present study, to address this issue, we examined in detail the behavior of post-crossing dI1 axons in mice, using the Atoh1 enhancer-based conditional expression system that enables selective and sparse labeling of individual dI1 axons, together with Hb9 and ChAT immunohistochemistry for precise identification of spinal motor neurons (MNs). We found unexpectedly that the post-crossing segment of dI1 axons later gave off collateral branches that extended laterally to invade motor columns. Interestingly, these collateral branches emerged at around the time when their primary growth cones initiated invasion into motor columns. In addition, although the length of the laterally growing collateral branches increased with age, the majority of them remained within motor columns. Strikingly, these collateral branches further gave rise to multiple secondary branche...Continue Reading

References

Jan 1, 1990·The Journal of Comparative Neurology·P E PhelpsJ E Vaughn
Jan 1, 1995·Annual Review of Neuroscience·S A Colamarino, M Tessier-Lavigne
Jun 8, 2001·Neuron·N A BerminghamH Y Zoghbi
Mar 20, 2002·Developmental Biology·Patricia E PhelpsTina Wong
Jun 8, 2002·Annual Review of Neuroscience·Ryuichi Shirasaki, Samuel L Pfaff
Dec 13, 2003·Science·Anna I LyuksyutovaYimin Zou
Apr 6, 2004·The Journal of Comparative Neurology·Stephanie Rebecca Kadison, Zaven Kaprielian
Mar 5, 2005·Nature Neuroscience·Dimitris BourikasEsther T Stoeckli
Jan 13, 2006·Developmental Biology·Hong-Lin SuYoshiki Sasai
Mar 31, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Edward S RuthazerHollis T Cline
Mar 31, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Martin P Meyer, Stephen J Smith
Apr 5, 2007·Nature Protocols·Tetsuichiro Saito
Aug 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stacey L ReeberZaven Kaprielian
Mar 20, 2009·The Journal of Comparative Neurology·Yumiko HatanakaMasayuki Masu
Dec 18, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nadine RabeKlas Kullander
Jan 16, 2010·Current Opinion in Neurobiology·Timothy A Evans, Greg J Bashaw
Jun 11, 2010·Cold Spring Harbor Perspectives in Biology·Barry J Dickson, Yimin Zou
Aug 20, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elena DomanitskayaEsther T Stoeckli
Aug 21, 2010·Current Opinion in Neurobiology·Alain Chédotal
May 4, 2011·Cellular and Molecular Life Sciences : CMLS·Homaira Nawabi, Valérie Castellani
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nozomi SakaiZaven Kaprielian
Mar 28, 2013·Annals of the New York Academy of Sciences·Anna Vallstedt, Klas Kullander

❮ Previous
Next ❯

Citations

Aug 7, 2018·The Journal of Comparative Neurology·Nuria García-MagroCarlos Avendaño
Jun 4, 2019·The Journal of Comparative Neurology·Alastair J TullochAlexander Jaworski

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.