Post-meiotic gene activity in spermatogenesis of the mouse

Cell Differentiation
A d'AgostinoV Monesi

Abstract

Mouse male germ cells at middle-late pachytene and early spermatid stages obtained by velocity sedimentation at unit gravity in albumin gradients were labelled in culture with [3H]uridine. The newly synthesized RNAs extracted from polysomes of the 2 cell types were studied by sucrose gradient fractionation and poly(U) Sepharose chromatography. The results showed that round spermatids, as well as pachytene spermatocytes, synthesize both ribosomal and polyadenylated RNA molecules. Since these latter are engaged in polysomes they are presumably active messenger RNA molecules.

References

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Citations

Sep 1, 1983·Cell Differentiation·A D'AgostinoR Geremia
Mar 1, 1979·The Journal of Cell Biology·M Parvinen, L M Parvinen
Feb 1, 1984·The Journal of Cell Biology·G L Gerton, C F Millette
Jan 1, 1983·Gastrointestinal Radiology·S DallemandJ Farman
Mar 1, 1981·European Journal of Pediatrics·G WeissbachM Domula
Apr 1, 1986·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·J C DavidJ Signoret

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