Post-mitotic role of nucleostemin as a promoter of skeletal muscle cell differentiation.

Biochemical and Biophysical Research Communications
Hiroyuki HiraiNobuaki Kikyo

Abstract

Nucleostemin (NS) is a nucleolar protein abundantly expressed in a variety of proliferating cells and undifferentiated cells. Its known functions include cell cycle regulation and the control of pre-rRNA processing. It also has been proposed that NS has an additional role in undifferentiated cells due to its downregulation during stem cell differentiation and its upregulation during tissue regeneration. Here, however, we demonstrate that skeletal muscle cell differentiation has a unique expression profile of NS in that it is continuously expressed during differentiation. NS was expressed at similar levels in non-proliferating muscle stem cells (satellite cells), rapidly proliferating precursor cells (myoblasts) and post-mitotic terminally differentiated cells (myotubes and myofibers). The sustained expression of NS during terminal differentiation is necessary to support increased protein synthesis during this process. Downregulation of NS inhibited differentiation of myoblasts to myotubes, accompanied by striking downregulation of key myogenic transcription factors, such as myogenin and MyoD. In contrast, upregulation of NS inhibited proliferation and promoted muscle differentiation in a p53-dependent manner. Our findings provi...Continue Reading

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Citations

Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Maximina H YunJeremy P Brockes
Jul 11, 2012·Stem Cells and Development·Haruhiko ShugoAtsushi Hirao
Sep 5, 2012·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·Yu V Markitantova, R D Zinovieva
Oct 20, 2010·The Journal of Cell Biology·Hiroyuki HiraiAtsushi Asakura
Jul 28, 2013·The American Journal of Pathology·Noriyuki UemaAtsushi Hirao

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