Post-mortem changes in human central nervous tissue and the effects on quantitation of nucleic acids and enzymes

The Histochemical Journal
D M MannJ S Davies

Abstract

A study of post-mortem changes in human central nervous tissue has shown that within 100 h of death, no significant change occurs in the amount of nerve cell DNA and nucleolar RNA nor in some membrane-associated enzymes such as succinate dehydrogenase, NADH and NADPH diaphorase, and cytochrome oxidase. Low molecular weight RNA species, probably transfer and messenger RNA are quickly lost, but there is little alteration in ribosomal RNA content. Cytoplasmic enzymes show variable changes; phosphofructokinase activity is rapidly decreased; hexokinase is unaltered but lactate dehydrogenase, pyruvate kinase and glucose-6-phosphate dehydrogenase initially show increases in activity which subsequently decline. Oxygen uptake diminishes quickly. These findings indicate that mechanical alterations in cell structure, following death, render organelles physiologically ineffective long before any significant changes in certain constituent biochemicals are detected. This report emphasizes the great importance necessary in the selection of appropriately time matched post-mortem tissues if accurate comparative studies of many of the cells constituents are to be made.

References

Mar 1, 1977·Journal of Neuropathology and Experimental Neurology·D M MannC M Barton
Feb 1, 1973·Journal of the Neurological Sciences·D M Mann, P O Yates
Feb 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J R Shea
May 1, 1974·British Medical Bulletin·W E Watson
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE
Mar 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M S BURSTONE
Jan 1, 1960·Virchows Archiv Für Pathologische Anatomie Und Physiologie Und Für Klinische Medizin·G MAJNOT E THOMPSON

Citations

Apr 1, 1985·The Histochemical Journal·D Hopwood
Jan 1, 1993·International Journal of Legal Medicine·T Gos, S Raszeja
Jan 1, 1983·Neurochemistry International·J A Hardy, Peter R Dodd
Sep 27, 2003·Journal of Biotechnology·Natalia A SkrypinaRobert Sh Beabealashvilli
Jan 1, 1979·Neuropathology and Applied Neurobiology·D Naber, H G Dahnke
Oct 27, 2009·Neurochemical Research·Zsolt KovácsKatalin A Kékesi
Sep 1, 1988·Cortex; a Journal Devoted to the Study of the Nervous System and Behavior·A R MayesA Pickering
Jan 1, 1980·Pathology, Research and Practice·M Oehmichen, M Gencic
Dec 19, 2006·Experimental and Molecular Pathology·Neera V Gopee, Paul C Howard
Apr 16, 2003·Journal of Neurochemistry·Matthew R HyndPeter R Dodd
Aug 1, 1982·Journal of Neurochemistry·T S BrannanM Bernstein
May 1, 1982·Neuropathology and Applied Neurobiology·D M Mann

Related Concepts

Brain
Brain Chemistry
Cerebellum
DNA, Double-Stranded
Glucosephosphate Dehydrogenase
Hexokinase 2
Lactate Dehydrogenase
Oxygen Consumption
6-Phosphofructokinase
Livor Mortis

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