Post-mortem in situ stability of serum markers of cerebral damage and acute phase response.

International Journal of Legal Medicine
Benjamin OndruschkaJan Dreßler

Abstract

The aim of the given study was to test the in situ stability of biochemical markers of cerebral damage and acute phase response in the early post-mortem interval to assess their usability for forensic pathology. A monocentric, prospective study investigated post-mortem femoral venous blood samples at four time points obtained within 48 h post-mortem starting at the death of 20 deceased, using commercial immunoassays for the ten parameters: S100 calcium-binding protein B (S100B), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), brain-derived neurotrophic factor (BDNF), interleukin-6 (IL-6), C-reactive protein (CRP), procalcitonin (PCT), ferritin, soluble tumor necrosis factor receptor type 1 (sTNFR1), and lactate dehydrogenase (LDH). Significant changes in serum levels were observed only later than 2 h after death for all markers. Inter-laboratory comparability was high, and intra-assay precision was sufficient for most markers. Most of the biomarker levels depended on the severity of hemolysis and lipemia but were robust against freeze-thaw cycles. Serum levels increased with longer post-mortem intervals for S100B, NSE, ferritin, sTNFR1, and LDH (for all p < 0.001) but decreased over this period for CRP (p...Continue Reading

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Citations

Mar 4, 2021·Medicine, Science, and the Law·Jack GarlandRexson Tse
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Meihui TianHao Pang
Apr 26, 2021·Legal Medicine·Carolin EdlerBenjamin Ondruschka
May 1, 2021·Microorganisms·Francesca ServadeiAlessandro Mauriello
May 7, 2021·International Journal of Legal Medicine·J GeissenbergerPeter Steinbacher
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Enrica RosatoCristian D'Ovidio
Jun 12, 2021·International Journal of Legal Medicine·Johann ZwirnerBenjamin Ondruschka

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