Post-mortem semen cryopreservation and characterization in two different endangered gazelle species (Gazella gazella and Gazella dorcas) and one subspecies (Gazella gazelle acaiae)

Theriogenology
Joseph SaragustyAmir Arav

Abstract

Both Gazella gazella and Gazella dorcas are endangered species with continually dwindling population size, yet basic knowledge on their spermatozoa is missing. Semen collected post-mortem (PM) from the cauda epididymis of five adult gazelles (three Gazella gazella gazella, one Gazella gazella acaiae and one G. dorcas) was cryopreserved using directional freezing of large volumes (8 mL) with egg-yolk-free extender. Sperm size measurements and SYBR-14/propodium iodide (PI) viability stain validation for use in gazelles were conducted. Post-thaw characterization included motility, viability, acrosome damage evaluation, computerized motility characterization and morphology and sperm motility index (SMI) was calculated. Extracted sperm motility was 71.67+/-11.67% (mean+/-S.E.M.). Post-thaw motility ranged between 15% and 63%, viability was 57.49+/-3.24%, intact acrosome was detected in 63.74+/-2.6% (median 64.8%, upper/lower quartiles 71.79%, 61.82%), and normal morphology ranged between 41% and 63%. Motility characterization showed two sub-groups-highly active and progressively motile spermatozoa with SMI of 62.75+/-0.38 and low activity and poorly progressive with SMI of 46.16+/-1.53. Our results indicate that PM preservation of g...Continue Reading

References

Aug 23, 1986·The Veterinary Record·A ShimshonyD Dagan
Sep 1, 1985·Journal of Reproduction and Fertility·J M Cummins, P F Woodall
Aug 1, 1995·Biology of Reproduction·D L Garner, L A Johnson
Jan 1, 1995·Reproduction, Fertility, and Development·R O Davis, R J Siemers
Mar 11, 1998·Proceedings. Biological Sciences·E R RoldanM Gomendio
Dec 3, 1998·Animal Reproduction Science·K L GoodroweW T Waddell
Jun 14, 2000·Animal Reproduction Science·M Yoshida
Jan 5, 2002·Theriogenology·S P Leibo, N Songsasen
May 16, 2002·Theriogenology·H IkedaH Kaneko
Mar 11, 2003·Reproduction, Nutrition, Development·Amir AravHaim Gacitua
Mar 24, 2004·Animal Reproduction Science·L H GrahamM M Buhr

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Citations

Mar 14, 2013·PloS One·Robert HermesThomas B Hildebrandt
Apr 15, 2008·The Journal of Reproduction and Development·Yutaka FukuiKentaro Okabe
Nov 11, 2008·The Journal of Reproduction and Development·Mami HiwasaYutaka Fukui
Dec 7, 2013·Reproduction, Fertility, and Development·Amir Arav, Joseph Saragusty
Feb 18, 2016·Animal Reproduction Science·Amir Arav, Joseph Saragusty
Jan 30, 2016·Reproductive Biology and Endocrinology : RB&E·Suhee KimYuksel Agca
Mar 21, 2009·Theriogenology·J SaragustyA Arav
Apr 12, 2016·Theriogenology·M T Prieto-PablosJ Saragusty
Nov 15, 2008·Theriogenology·R HermesT B Hildebrandt
Dec 26, 2008·Animal Reproduction Science·Joseph SaragustyAmir Arav
Dec 30, 2008·Animal Reproduction Science·Joseph SaragustyRobert Hermes
Jul 1, 2008·Animal Reproduction Science·Robert HermesDetlef Rath
Jul 14, 2009·Biotechnology and Bioengineering·Joseph SaragustyAmir Arav
Nov 3, 2007·Reproduction in Domestic Animals = Zuchthygiene·J SaragustyA Arav
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·A Arav, D Natan
Aug 22, 2006·Animal Reproduction Science·S M H Andrabi, W M C Maxwell
Sep 10, 2015·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·María Teresa Prieto PablosRobert Hermes
May 16, 2014·Cryobiology·Antônio Cavalcante Mota FilhoLúcia Daniel Machado da Silva
Jun 2, 2016·Reproduction, Fertility, and Development·Budhan S Pukazhenthi
Apr 11, 2007·The Journal of Reproduction and Development·Yutaka FukuiMami Hiwasa
Feb 9, 2018·Zoological Science·Sebastian J CharltonJulie Strand

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