PMID: 6969697Nov 1, 1980Paper

Post-repair DNA damage in X-irradiated cultured human tumour cells

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
P M Kanter, H S Schwartz

Abstract

In three human cells lines, X-irradiation damage to DNA is quantitatively repaired in 2 hours or less. At 4 hours following single doses of 1000 rad or less, delayed or latent damage to the DNA appears in CCRF-CEM and 8402 leukaemia cells; HeLa cells under these conditions demonstrate comparatively little such post-repair DNA damage. With larger doses (2000-8000 rad), DNA in HeLa cells also exhibits post-repair damage. This damage is in the form of small acid-precipitable, double-stranded DNA fragments, and is demonstrated by batch elution from hydroxylapatite and alkaline and neutral sucrose sedimentation velocity techniques. The appearance of delayed DNA damage may be an early, critical expression of radiation-induced cytotoxic progression because the lesions are dose-dependent and are apparently not repaired.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T R ButtM E Smulson
Jun 1, 1978·Journal of the National Cancer Institute·J MinowadaE Tatsumi
Aug 1, 1977·The Australian Journal of Experimental Biology and Medical Science·M M DonJ F Kerr
Aug 1, 1979·Analytical Biochemistry·P M Kanter, H S Schwartz
Jul 1, 1963·Biophysical Journal·E BURGI, A D HERSHEY

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.