Post-Synthetic Reduction of Pectin Methylesterification Causes Morphological Abnormalities and Alterations to Stress Response in Arabidopsis thaliana

Plants
Nathan T ReemOlga A Zabotina

Abstract

Pectin is a critical component of the plant cell wall, supporting wall biomechanics and contributing to cell wall signaling in response to stress. The plant cell carefully regulates pectin methylesterification with endogenous pectin methylesterases (PMEs) and their inhibitors (PMEIs) to promote growth and protect against pathogens. We expressed Aspergillus nidulans pectin methylesterase (AnPME) in Arabidopsis thaliana plants to determine the impacts of methylesterification status on pectin function. Plants expressing AnPME had a roughly 50% reduction in methylester content compared with control plants. AnPME plants displayed a severe dwarf phenotype, including small, bushy rosettes and shorter roots. This phenotype was caused by a reduction in cell elongation. Cell wall composition was altered in AnPME plants, with significantly more arabinose and significantly less galacturonic acid, suggesting that plants actively monitor and compensate for altered pectin content. Cell walls of AnPME plants were more readily degraded by polygalacturonase (PG) alone but were less susceptible to treatment with a mixture of PG and PME. AnPME plants were insensitive to osmotic stress, and their susceptibility to Botrytis cinerea was comparable to...Continue Reading

References

May 1, 1972·Analytical Biochemistry·M Lever
Sep 2, 1996·Carbohydrate Research·P PellerinM A O'Neill
Oct 13, 1998·Molecular Plant-microbe Interactions : MPMI·A ten HaveJ A van Kan
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Sep 16, 2000·Carbohydrate Research·J M DenèsJ F Drilleau
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Odile Valette-ColletMartine Boccara
Sep 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Louise JonesSimon J McQueen-Mason
Feb 12, 2004·Biochimica Et Biophysica Acta·A GiovaneL Camardella
Jun 18, 2004·Molecular Cell·Matthew W Jones-Rhoades, David P Bartel
Jul 20, 2004·The Plant Cell·Ramanjulu Sunkar, Jian-Kang Zhu
Sep 1, 2004·Carbohydrate Research·Oskar Markovic, Stefan Janecek
Dec 25, 2004·Science·Chris SomervilleHeather Youngs
Jul 7, 2005·The Plant Journal : for Cell and Molecular Biology·Ilona KarsJan A L van Kan
Nov 2, 2005·Nature Reviews. Molecular Cell Biology·Daniel J Cosgrove
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Nélida BritoCeledonio González
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Bruce D KohornNicole Byers
Jun 13, 2006·Plant Physiology·Regina SchuheggerErich Glawischnig
Jul 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefan BauerChris R Somerville
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Thomas DuvetterAnn Van Loey
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jul 21, 2009·Carbohydrate Research·Kerry Hosmer Caffall, Debra Mohnen
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vincenzo LionettiFelice Cervone
Dec 22, 2010·Molecular Plant-microbe Interactions : MPMI·Alessandro RaiolaDaniela Bellincampi
Jan 20, 2011·Biotechnology Letters·Alicia Lara-MárquezHoracio Cano Camacho
Jul 26, 2011·Applied Microbiology and Biotechnology·Joost van den Brink, Ronald P de Vries
Sep 13, 2011·Plant Molecular Biology·Gennady PogorelkoOlga A Zabotina
Jan 10, 2012·Annual Review of Plant Biology·Sebastian WolfHerman Höfte

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
Assay

Software Mentioned

ImageJ

Related Concepts

Related Feeds