Post-Thaw Viable CD45+ Cells and Clonogenic Efficiency are Associated with Better Engraftment and Outcomes after Single Cord Blood Transplantation in Adult Patients with Malignant Diseases

Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation
Nerea CastilloSergio Querol

Abstract

The quantity of cells is widely accepted as the main factor influencing the outcome after umbilical cord blood transplantation (UCBT) however, the quality of the cord blood units (CBUs) has been less studied. In order to determine the impact of qualitative variables in UCBT outcomes, we conducted a multicenter retrospective study in adult patients with hematological malignancies who underwent single UCBT after a common myeloablative conditioning regimen. One hundred and ten patients from 3 institutions [median age, 35 years (range 18-55)] were included. Quantitative (TNC and total CD34+cells) and qualitative variables [viable CD45+ (vCD45+), vCD34+ and clonogenic efficiency [(CLONE), quotient of post-thaw colony-forming units (CFU)] and pre-freeze CD34+ cells predicted engraftment in univariate analysis however, only 2 qualitative variables remained significant in the multivariate analysis. Infusion of more than 2 × 10(7) post-thaw vCD45+ cells per kilogram was significantly associated with faster neutrophil (P = .01), platelet engraftment (P = .01), higher disease-free (P = .01) and overall survival (0.02). In addition, CLONE ≥ 20% predicted a faster neutrophil (P = .005), platelet engraftment (P = .01) and contributed to decr...Continue Reading

References

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Citations

Apr 4, 2016·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Nerea CastilloSergio Querol
May 5, 2016·Transfusion·Marc CloutierSonia Néron
Feb 11, 2021·International Journal of Immunogenetics·Ann-Margaret LittlePaul A Wright
Apr 21, 2021·British Journal of Haematology·Sergio QuerolAlejandro Madrigal
Jul 17, 2021·Bone Marrow Transplantation·Gaku KondoUNKNOWN Japanese Red Cross Kanto-Koshinetsu Cord Blood Bank

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