Abstract
Expression of epidermal growth factor receptor (EGF-R) antisense RNA results in a drastic reduction of EGF-R levels in the human carcinoma KB cell line and induces a reversion of their transformed phenotype (Moroni, M. C., Willingham, M. C., and Beguinot, L. (1992) J. Biol. Chem. 267, 2714-2722). We used parental and EGF-R antisense KB clones as a genetic system to study, in the same cell line, the role of transforming growth factor alpha (TGF-alpha) in the establishment and maintenance of the transformed phenotype. KB cells produce TGF-alpha mRNA, and their conditioned medium is able to sustain growth of antisense cells, mimicking the effect of exogenous EGF or TGF-alpha. In antisense cells there is a marked reduction of TGF-alpha mRNA steady-state levels. In addition, the decrease in TGF-alpha parallels the levels of residual EGF-R in the various antisense clones, indicating a direct correlation between receptors and growth factor levels. The addition of exogenous TGF-alpha (10 ng/ml) to antisense clones induces TGF-alpha levels. The half-life of TGF-alpha mRNA is 40-60 min in antisense cells and more than 8 h in parental KB cells, as determined by actinomycin D decay curves. This result indicates a predominant regulation of ...Continue Reading
References
Jan 1, 1992·Advances in Cancer Research·R Derynck
Feb 22, 1991·Science·S HigashiyamaM Klagsbrun
Apr 1, 1991·International Journal of Cancer. Journal International Du Cancer·N TakekuraE Tahara
Jan 1, 1990·Japanese Journal of Cancer Research : Gann·K YoshidaE Tahara
Aug 2, 1987·Nature·R J CoffeyM R Pittelkow
Jan 1, 1989·Molecular and Cellular Biology·T M BrowderA W Nienhuis
May 1, 1989·British Journal of Cancer·K ImanishiK Abe
Aug 1, 1989·Carcinogenesis·R ToddD T Wong
Jun 1, 1989·Molecular Endocrinology·S G MuellerJ E Kudlow
Sep 29, 1989·Science·C E DunbarA W Nienhuis
Mar 15, 1989·International Journal of Cancer. Journal International Du Cancer·L T MaldenA W Burgess
Mar 11, 1985·Nucleic Acids Research·P FortJ M Blanchard
Aug 4, 1985·Nature·F CuttittaJ D Minna
Jun 10, 1988·Nucleic Acids Research·Y JinnoI Pastan
May 1, 1987·The Journal of Experimental Medicine·S J WarnerP Libby
Aug 20, 1987·Nature·Y PaulssonB Westermark
Dec 4, 1987·Science·T J VeluD R Lowy
Dec 24, 1987·Cell·P P Di FioreS A Aaronson
Jan 1, 1983·DNA·G CathalaJ D Baxter
Oct 9, 1980·The New England Journal of Medicine·M B Sporn, G J Todaro
Oct 4, 1995·Biochemical and Biophysical Research Communications·I SiddiqiM Korc
Aug 18, 1995·The Journal of Biological Chemistry·M MilosoL Beguinot
Oct 15, 1993·Gene·J F QianE May
Citations
May 12, 2000·The Laryngoscope·J Rubin GrandisS D Drenning
Jul 12, 2011·Physiological Reviews·Shom GoelRakesh K Jain
Oct 14, 1998·The Journal of Clinical Investigation·J R GrandisD J Tweardy
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J R GrandisJ D Kim
Feb 16, 2008·Reproductive Sciences·Tai-Ho HungT'sang-T'ang Hsieh
Oct 7, 2003·Oncogene·Sylvie MénardElda Tagliabue
Mar 22, 2002·Nature·Yotaro IzumiRakesh K Jain
May 13, 2010·Histopathology·Fabrício F T BarrosAndrew R Green
Jun 5, 1998·The American Journal of Physiology·X L YaoJ H Shelhamer
May 29, 2003·The Journal of Biological Chemistry·Sichuan XiJennifer Rubin Grandis
Apr 5, 2007·The Biochemical Journal·Miguel M MurilloIsabel Fabregat