Post-transcriptional control regulates transforming growth factor alpha in the human carcinoma KB cell line.

The Journal of Biological Chemistry
G NicoliniL Scotto

Abstract

Expression of epidermal growth factor receptor (EGF-R) antisense RNA results in a drastic reduction of EGF-R levels in the human carcinoma KB cell line and induces a reversion of their transformed phenotype (Moroni, M. C., Willingham, M. C., and Beguinot, L. (1992) J. Biol. Chem. 267, 2714-2722). We used parental and EGF-R antisense KB clones as a genetic system to study, in the same cell line, the role of transforming growth factor alpha (TGF-alpha) in the establishment and maintenance of the transformed phenotype. KB cells produce TGF-alpha mRNA, and their conditioned medium is able to sustain growth of antisense cells, mimicking the effect of exogenous EGF or TGF-alpha. In antisense cells there is a marked reduction of TGF-alpha mRNA steady-state levels. In addition, the decrease in TGF-alpha parallels the levels of residual EGF-R in the various antisense clones, indicating a direct correlation between receptors and growth factor levels. The addition of exogenous TGF-alpha (10 ng/ml) to antisense clones induces TGF-alpha levels. The half-life of TGF-alpha mRNA is 40-60 min in antisense cells and more than 8 h in parental KB cells, as determined by actinomycin D decay curves. This result indicates a predominant regulation of ...Continue Reading

References

Jan 1, 1992·Advances in Cancer Research·R Derynck
Apr 1, 1991·International Journal of Cancer. Journal International Du Cancer·N TakekuraE Tahara
May 1, 1989·British Journal of Cancer·K ImanishiK Abe
Aug 1, 1989·Carcinogenesis·R ToddD T Wong
Mar 15, 1989·International Journal of Cancer. Journal International Du Cancer·L T MaldenA W Burgess
Jun 10, 1988·Nucleic Acids Research·Y JinnoI Pastan
May 1, 1987·The Journal of Experimental Medicine·S J WarnerP Libby
Oct 9, 1980·The New England Journal of Medicine·M B Sporn, G J Todaro
Oct 4, 1995·Biochemical and Biophysical Research Communications·I SiddiqiM Korc

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Citations

Jul 12, 2011·Physiological Reviews·Shom GoelRakesh K Jain
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J R GrandisJ D Kim
Feb 16, 2008·Reproductive Sciences·Tai-Ho HungT'sang-T'ang Hsieh
Oct 7, 2003·Oncogene·Sylvie MénardElda Tagliabue
Mar 22, 2002·Nature·Yotaro IzumiRakesh K Jain
May 29, 2003·The Journal of Biological Chemistry·Sichuan XiJennifer Rubin Grandis

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