Post-Transcriptional Dysregulation of microRNA and Alternative Polyadenylation in Colorectal Cancer

Frontiers in Genetics
Zhanrui MaoYani Kang

Abstract

Colorectal cancer (CRC) is one of the leading causes of cancer death worldwide. microRNAs (miRNAs) repress gene expression by binding to complementary sequences in the 3' untranslated region (3'UTR) of target mRNAs. Alternative polyadenylation (APA) are relevant to the variability of the 3'UTR of mRNA. However, the posttranscriptional dysregulation of miRNAs and APA in CRC are poorly understood. In this study, we conducted small RNA sequencing to identify differentially expressed miRNAs (DERs) and their target genes. Function analysis on DER-target genes can explain the regulation roles of miRNAs in CRC. The mutual regulation of miRNAs and APA was analyzed by combining miRNA data to 3'UTR alteration using 3' termini of polyadenylated RNAs sequencing (3T-seq) technique, and this was validated using TCGA gene expression data. Our results showed 64 significant differentially expressed miRNAs (DERs) in CRC patients. Their target genes were related to cell adhesion and transcription regulation and were prevailingly involved in the CRC-related pathway. Integrative analysis of the miRNA and APA profile revealed 16 DERs were correlated with 12 polyadenylation factors, and six of them were significantly differently expressed in CRC. We ...Continue Reading

References

Mar 7, 2003·The New England Journal of Medicine·Henry T Lynch, Albert de la Chapelle
Aug 1, 2008·Nature·Matthias SelbachNikolaus Rajewsky
Mar 23, 2010·Lancet·David CunninghamNaureen Starling
Mar 29, 2012·Genome Research·Adnan DertiTomas Babak
Apr 21, 2012·Journal of Thyroid Research·Yoshiyuki BanTsutomu Hirano
Jun 19, 2013·Nature Reviews. Genetics·Ran ElkonReuven Agami
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
May 23, 2015·Nature Reviews. Cancer·Shuibin Lin, Richard I Gregory
Jun 13, 2015·BioMed Research International·Jian WangYinxue Yang
Apr 15, 2016·Molecular Cancer Research : MCR·Ayse Elif Erson-Bensan, Tolga Can
Jan 17, 2017·Journal of Cellular Physiology·Abdullah MoridikiaJafar Salimian
Oct 3, 2017·Carcinogenesis·Chioniso P Masamha, Eric J Wagner
Oct 14, 2017·Pathology International·Noriyuki NakanoMasayuki Noguchi
Nov 11, 2017·Nucleic Acids Research·Chih-Hung ChouHsien-Da Huang
Dec 12, 2017·Cell·Abraham S WeintraubRichard A Young
Jan 13, 2018·International Journal of Cancer. Journal International Du Cancer·Levon M Khachigian
Oct 17, 2019·Przegla̜d gastroenterologiczny·Prashanth RawlaAdam Barsouk

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Datasets Mentioned

BETA
GSE130084
E-MTAB-6403

Methods Mentioned

BETA
RNA-seq
3T-seq
CLASH
histone acetylation

Software Mentioned

clusterProfiler
ENCORI ( Encyclopedia Interactomes )
GEPIA
Fastqc
Cytoscape
edgeR
Integrative Genomics Viewer ( IGV )
Bowtie2

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