Post-transcriptional gene silencing of GBSSI in potato: effects of size and sequence of the inverted repeats.

Plant Molecular Biology
H J B HeilersigR G Visser

Abstract

In the past, silencing of granule-bound starch synthase (GBSSI) in potato was achieved by antisense technology, where it was observed that inclusion of the 3' end of the GBSSI coding region increased silencing efficiency. Since higher silencing efficiencies were desired, GBSSI inverted repeat constructs were designed and tested in potato. First, large inverted repeats comprising the 5' and the 3' half of the GBSSI cDNA were tested. The 5' IR construct gave a significantly higher silencing efficiency than the 3' IR construct. Since it was not known whether the observed difference was due to the sequence or the orientation of the inverted repeat, the GBSSI cDNA was divided into three regions, after which each region was tested in small inverted repeats in two orientations. To this end large numbers of independent transformants were produced for each construct. The results suggested that there was no effect of inverted repeat orientation on silencing efficiency. The percentage of transformants showing strong inhibition varied from 48% for a 3'-derived construct to 87% for a 5' as well as a middle region-derived construct. Similar to the large inverted repeats, the 3' sequences induced the least efficient silencing implying that th...Continue Reading

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Citations

Feb 3, 2006·Molecular Genetics and Genomics : MGG·Berlinda H J B HeilersigRichard G F Visser
Jan 8, 2010·Briefings in Functional Genomics·Karen M McGinnis
Jul 3, 2013·PloS One·Robin P HuibersYuling Bai
Aug 7, 2012·International Journal of Food Sciences and Nutrition·Rajan Katoch, Neelam Thakur
Sep 18, 2009·Journal of Plant Physiology·Takashi NakatsukaMasahiro Nishihara
Jul 5, 2008·Fungal Genetics and Biology : FG & B·Audrey M V Ah-FongHoward S Judelson
May 19, 2009·Molecular Plant-microbe Interactions : MPMI·Anoma A LokossouEdwin A G van der Vossen
Jun 10, 2010·Bioorganicheskaia khimiia·E B RukavtsovaIa I Bur'ianov
Nov 30, 2019·Scientific Reports·Ida Elisabeth JohansenBent Larsen Petersen
Jun 3, 2021·International Journal of Molecular Sciences·Tosin Victor AdegokeJian Zhang

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