PMID: 6108552Jul 25, 1980Paper

Post-transcriptional regulation of messenger abundance in rat liver and hepatoma

Nucleic Acids Research
H Jacobs, G D Birnie

Abstract

Saturation hybridisation with labelled single-copy DNA shows that polysomal poly(A)+ RNA of rat liver and of a minimum-deviation rat hepatoma cell-line (HTC) have similar total complexities and that few sequences are specific to either cell-type. Hybridisation kinetics of polysomal cDNAs with template and heterologous cell RNAs indicate that a proportion of liver messengers are at greatly reduced abundance in the hepatoma, but not the converse. Hybridisations using fractionated cDNAs enriched for abundant polysomal sequences confirm these findings: on average, abundant liver mRNAs are about 100-fold rarer in hepatoma, whereas abundant hepatoma mRNAs are only 5-fold rarer in liver. This pattern is also implied by cell-free translation of polysomal poly(A)+ RNAs. The reactions of total and fractionated polysomal cDNA probes with poly(A)+ nuclear RNA indicate that there is much less disparity in abundance of these sequences at nuclear level, implying that the differences arise, at least in part, post-transcriptionally. We interpret the altered mRNA abundances in the hepatoma in terms both of its decreased functional specialisation and its ability to proliferate.

References

Nov 17, 1978·Biochimica Et Biophysica Acta·T Graf, H Beug
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·J PaulG D Birnie
Oct 27, 1978·Biochimica Et Biophysica Acta·M Greaves, G Janossy
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Citations

Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T M GressH Lehrach
May 22, 1981·Molecular Biology Reports·G D BirnieJ Paul
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M YamamotoH Endo
Jan 1, 1982·European Journal of Biochemistry·H Jacobs, G D Birnie
Jan 14, 1983·Biochemical and Biophysical Research Communications·C Icard-LiepkalnsT E Webb
Apr 1, 1984·Journal of the Royal Society of Medicine·G D BirnieA M Warnock
Mar 1, 1982·Molecular and Cellular Biology·Y G CapetanakiA Alonso

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