Post-transcriptional regulator Rbm47 elevates IL-10 production and promotes the immunosuppression of B cells

Cellular & Molecular Immunology
Yinxiang WeiZhijian Cai

Abstract

Regulatory B cells (Bregs) are a functionally defined B cell subset, and IL-10 is crucial for the suppressive functions of Bregs. However, little is known regarding how IL-10 production is regulated in B cells. To explore the mechanisms by which IL-10 is regulated in B cells, we used mRNA microarrays to screen for molecules that are upregulated in IL-10-producing B cells and identified RNA-binding motif protein 47 (Rbm47) as a post-transcriptional regulator. Rbm47 was found to promote IL-10 production in B cells. We found that Rbm47 promotes the stability of IL-10 mRNA by binding to AU-rich elements in the 3' untranslated region of Il10 mRNA. In addition, we demonstrated that the overexpression of Rbm47 enabled B cells to facilitate Foxp3+ regulator T-cell induction and reduce the severity of DSS-induced ulcerative colitis. Taken together, these results suggest that Rbm47 plays an important role in regulating IL-10 at the post-transcriptional level, thus promoting the regulatory functions of B cells. The findings presented in this study not only increase our understanding of the post-translational regulation of IL-10 in B cells but also identify a novel strategy for the potential application of Bregs.

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Citations

Aug 30, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Long-Feng LuShun Li
Feb 14, 2021·Science China. Life Sciences·Jing WenBing Sun
Jul 19, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Xiao-Chen XuChun-Ling Luo
Sep 10, 2021·Molecular and Cellular Biochemistry·Pavan Kumar Mysuru ShivalingappaSharmila A Bapat

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Methods Mentioned

BETA
PMA
immunoprecipitation
PCR
Assay
FACS
ELISA
RIP
pulled down

Software Mentioned

GraphPad Prism
CellQuest
Bregs
GraphPad

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