PMID: 6991124Apr 1, 1980Paper

Post-transcriptional regulatory mutants in a ribosomal protein-RNA polymerase operon of E. coli

Cell
N FiilP P Dennis

Abstract

A high copy number plasmid that carries the promoter PJ, intact rplJ and a deletion of the 3' terminal portion of rplL is detrimental to the growth of the host bacterium. Six independent point mutations on the plasmid that overcome this detriment have been isolated. Nucleotide sequence analysis demonstrates that all six mutants are single base pair alterations, occur within the leader region of the rplJ operon and are well removed from the presumed position of the primary promoter, PJ. These mutant plasmids exhibit normal transcription of rplJ-rplL but do not translate rplJ messenger RNA to yield plasmid-specified L10 ribosomal protein. We suggest that these mutations define a regulatory region within the leader sequence of the RNA transcript that serves to modulate the translational efficiency of rplJ messenger RNA.

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Citations

Jan 1, 1982·Molecular & General Genetics : MGG·J HahnD Dubnau
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A PeattieH F Noller
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N RobakisH Weissbach
Jul 1, 1988·The Journal of Cell Biology·M Kozak
Dec 21, 1981·Nucleic Acids Research·C SquiresC L Squires
Mar 1, 1994·Molecular Microbiology·J Chow, P P Dennis
May 18, 2004·RNA·Helen J MerianosPeter B Moore
Jan 1, 1981·Archives of Biochemistry and Biophysics·N BrotH Weissbach
Apr 20, 1987·Journal of Molecular Biology·W L Downing, P P Dennis
Jun 1, 1983·Microbiological Reviews·B J Bachmann
Dec 1, 1982·Journal of Bacteriology·I HuiP Dennis
Dec 1, 1986·Microbiological Reviews·T C KingD Schlessinger
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Jul 20, 1987·Journal of Molecular Biology·I C DeckmanM S Thomas

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