PMID: 6404626Apr 15, 1983Paper

Post-translational fate of variant MOPC 315 lambda chains in Xenopus oocytes and mouse myeloma cells

European Journal of Biochemistry
G ValleA Colman

Abstract

The post-translational fates of three immunoglobulin lambda chain variants of MOPC 315 were investigated in mouse plasmacytoma cell lines and in mRNA-microinjected Xenopus oocytes. Quite unexpectedly we found that one non-secretory variant chain (lambda-43) underwent extensive post-translational N-glycosylation: however the presence of the oligosaccharide moiety did not account for the nonsecretory phenotype nor did it affect the rate of degradation of this lambda chain. Another variant chain (lambda-47) at first believed to be non-secretory, was found to be secreted from oocytes at a very low level, but mostly as a lambda-lambda dimer. In myeloma cells a low level of lambda-47 chain was secreted and again lambda-lambda dimers were the favoured secretory form. The secretory lambda-48 chain also formed lambda-lambda dimers, whereas lambda-43, which was never secreted, was only found as a monomeric lambda chain in both oocytes and myeloma cells. A similar relationship between assembly and secretion was found when oocytes were coinjected with MOPC 21 heavy (gamma 1) chain mRNA and MOPC 315 lambda chain mRNAs. The wild type lambda chain (lambda-48) was able to assemble with the gamma chain in a covalently bound tetramer (gamma gamm...Continue Reading

References

Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·C MilsteinC D Wilde
Jul 1, 1979·Cell·A Colman, J Morser
Apr 1, 1976·European Journal of Immunology·G KöhlerC Milstein
Nov 1, 1977·The Journal of Experimental Medicine·A M Tartakoff, P Vassalli
Sep 27, 1972·Nature: New Biology·C MilsteinM B Mathews
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B E Roberts, B M Paterson
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Jul 22, 1982·Nature·A L BothwellD Baltimore
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G Köhler
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Oct 1, 1981·Archives of Biochemistry and Biophysics·J A Hanover, W J Lennarz
Oct 1, 1980·European Journal of Biochemistry·C D LaneB J Miflin

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Citations

Jan 1, 1990·The International Journal of Biochemistry·J J Heikkila
Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R F AldersonE T Butler
Jan 1, 1985·CRC Critical Reviews in Biochemistry·H Soreq
Nov 1, 1987·European Journal of Immunology·A AlanenR M Franklin

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