Post-translational modifications in the nuclear region of young, aged, and cataract human lenses

Journal of Proteome Research
Peter G Hains, Roger John Willis Truscott

Abstract

The urea-soluble proteins from the nucleus of two young, two aged, and two early-stage nuclear cataract lenses were subjected to tryptic digestion and analysis by 2D LC-MS/MS. Several novel post-translational modifications were identified. Deamidation was, by far, the most common modification. A number of differences were found in cataract compared to normal lenses, most notably an increase in the number of oxidized tryptophan residues. Semiquantitative analysis revealed that there appeared to be a trend toward increased levels of deamidation with age; however, there was no apparent increase upon the onset of nuclear cataract. This is in contrast to Trp oxidation, where an increase in the extent of modification was apparent in cataract lenses when compared to aged normal lenses. These findings suggest Trp oxidation may be involved in nuclear cataract development.

References

Feb 20, 1991·Journal of Molecular Biology·D Bordo, P Argos
Apr 1, 1969·Experimental Eye Research·R ClarkS Lerman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M H Garner, A Spector
Mar 17, 1995·Biochemical and Biophysical Research Communications·M Cherian, E C Abraham
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
May 15, 1997·Analytical Biochemistry·H C PotgieterW J Vermaak
May 1, 1997·Experimental Eye Research·J A AquilinaR J Truscott
Jan 10, 1998·Photochemistry and Photobiology·E L FinleyK L Schey
Nov 25, 1998·Protein Science : a Publication of the Protein Society·E L FinleyK L Schey
Apr 27, 1999·Progress in Retinal and Eye Research·B K Derham, J J Harding
Apr 15, 2000·The Journal of Biological Chemistry·J FengJ B Smith
Feb 22, 2001·Experimental Eye Research·K J LampiD M Kapfer
Aug 30, 2001·Experimental Gerontology·H Lindner, W Helliger
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·N E Robinson, A B Robinson
Nov 14, 2001·The Journal of Biological Chemistry·Andrew G PurkissChristine Slingsby
May 2, 2002·Biochimica Et Biophysica Acta·J A Aquilina, R J W Truscott
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J MacCossJohn R Yates
Feb 5, 2003·Experimental Eye Research·Joseph Horwitz
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Veniamin N LapkoJean B Smith
Oct 11, 2003·Experimental Eye Research·Peter G HainsRoger J W Truscott
Dec 24, 2003·Biochemistry·Rebecca J KapphahnDeborah A Ferrington
Dec 24, 2003·Investigative Ophthalmology & Visual Science·Ratna Gupta, Om P Srivastava
Aug 11, 2004·Progress in Biophysics and Molecular Biology·Hans BloemendalAnnette Tardieu
Jan 12, 2005·Experimental Eye Research·Durga SrikanthanChristine Slingsby
Apr 13, 2005·Journal of Proteome Research·Brian C SearleSrinivasa R Nagalla
Aug 27, 2005·Investigative Ophthalmology & Visual Science·Yuri V SergeevJ Fielding Hejtmancik
Aug 25, 2006·The Biochemical Journal·Heath EcroydJohn A Carver

❮ Previous
Next ❯

Citations

May 22, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Andor J KissRachael M Morgan-Kiss
Jan 22, 2010·Amino Acids·Anita J GrosvenorJolon M Dyer
Feb 17, 2010·Journal of Ocular Biology, Diseases, and Informatics·Phillip A WilmarthLarry L David
Sep 18, 2009·The Journal of Biological Chemistry·Kate L Moreau, Jonathan King
Mar 17, 2010·Rejuvenation Research·Roger John Willis Truscott
Jul 1, 2010·Rejuvenation Research·Xiangjia ZhuRoger J W Truscott
Jun 12, 2008·Redox Report : Communications in Free Radical Research·Alexandra NowakRüdiger Hardeland
Mar 13, 2014·Progress in Biophysics and Molecular Biology·Kirsten J LampiLarry L David
May 20, 2014·Progress in Biophysics and Molecular Biology·Eugene Serebryany, Jonathan A King
Dec 3, 2014·Biochemical and Biophysical Research Communications·Yanqin LiuTara L Pukala
Apr 24, 2012·Trends in Molecular Medicine·Kate L Moreau, Jonathan A King
May 24, 2011·Experimental Eye Research·Daniel R GouletJonathan A King
Mar 2, 2010·Experimental Eye Research·Magalie MichielStéphanie Finet
May 26, 2009·Biochimica Et Biophysica Acta·K Krishna Sharma, Puttur Santhoshkumar
Apr 9, 2009·Free Radical Biology & Medicine·Marilyn EhrenshaftRonald P Mason
Jan 13, 2009·Experimental Eye Research·Anastasia KorlimbinisRoger J W Truscott
Oct 7, 2008·Experimental Eye Research·Larry Takemoto, Christopher M Sorensen
Sep 2, 2008·Biochimica Et Biophysica Acta·Peter G Hains, Roger J W Truscott
Sep 1, 2015·Biochimica Et Biophysica Acta·Nicholas J RayJohn A Carver
Jun 24, 2008·Protein Science : a Publication of the Protein Society·Takumi TakataKirsten J Lampi
May 21, 2013·Photochemistry and Photobiology·Nathaniel Schafheimer, Jonathan King
Jul 20, 2011·Journal of the Science of Food and Agriculture·Anita J GrosvenorJolon M Dyer
Oct 28, 2011·Mass Spectrometry Reviews·Z Kyselova
Jan 15, 2014·Protein Science : a Publication of the Protein Society·Sean D MoranMartin T Zanni
Jan 12, 2016·Physical Chemistry Chemical Physics : PCCP·P S SherinYu P Tsentalovich
Aug 5, 2015·Journal of the American Society for Mass Spectrometry·Shuzhen ZhengYukui Zhang
Apr 7, 2015·International Journal of Biological Macromolecules·Susmitnarayan ChaudhurySwagata Dasgupta
Jan 1, 2015·Nature Reviews. Disease Primers·Dennis LamDavid F Chang
Sep 5, 2013·Colloids and Surfaces. B, Biointerfaces·Pavan SunkireddySubhash C Yadav
Mar 19, 2016·Protein Science : a Publication of the Protein Society·Eugene SerebryanyJonathan A King
Aug 21, 2013·Biochemistry·Sean D MoranMartin T Zanni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.