Post-translational modifications influence IgE reactivity to the major allergen Phl p 1 of timothy grass pollen

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
A PetersenW M Becker

Abstract

Grass group I consists of very potent allergenic components which are found in the pollen of all temperate grasses. Several post-translational modifications are predicted from the cDNA data. The aim of this study was to identify sequential IgE-binding sites on the allergen Phl p 1 and to determine their influence on IgE reactivity. Based on cDNA data and microsequencing results we synthesized overlapping decapeptides covering the complete Phl p 1 molecule and tested them for immunological reactivity by means of the PEPSCAN technique. In a dot test we determined the frequency of IgE reactivities to post-translationally modified structures (hydroxylated proline residues, carbohydrate structure, and disulphide formations). Screening by overlapping peptides demonstrated an IgE binding site on the 10 N-terminal amino acids. Comprehensive studies showed that the two hydroxyproline residues of the native Phl p 1 allergen (at positions 5 and 8) and the N-glycan (at position 9) can result in an increased IgE reactivity; 3.3% of the sera exclusively bound to the hydroxyproline bearing peptide, while only 0.4% bound to the proline containing peptide. With regard to glycosylation, we estimated that 20% of sera recognized protein and carboh...Continue Reading

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Citations

Jul 14, 2012·Plant Physiology·Zhang YangBent L Petersen
Sep 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Neela H YennawarDaniel J Cosgrove
May 8, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W R Thomas, W Smith
Feb 26, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R SuckH Fiebig
Feb 7, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P IacovacciC Pini
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May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·U SeitzerJ Ahmed
May 23, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P RupaY Mine
Jun 27, 2013·Proteomics·Virgínia Maria Ferreira ResendeAndrej Shevchenko
Dec 9, 2014·The Journal of Allergy and Clinical Immunology·Hervé BernardStéphane Hazebrouck
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Sep 23, 2014·PloS One·Marcela Caleffi da Costa Lima CaniattiLuiza Tamie Tsuneto
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May 30, 2021·Molecular Immunology·Azahara González-RuizJerónimo Carnés
Jun 8, 2021·Molecular Immunology·Nicolas VisezPascal Poncet

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