PMID: 7541897Mar 1, 1995Paper

Post-translational modifications of apolipoprotein A-I and Po proteins in the avian peripheral nerve

Neurochemical Research
J LemieuxC Mezei

Abstract

Apolipoprotein A-I (apo A-I), a soluble lipid transporter, and Po, the major glycoprotein of myelin, are actively synthesized during myelination. To explore the status of post-translational modifications of these proteins in the avian PNS during rapid myelination, endoneurial slices from one day old chick sciatic nerves were incubated with various radioactive precursors that could serve as indicators of such processes. The proteins were isolated from the incubation medium (secreted fraction), the 1% Triton-X-100-soluble intracellular-endoneurial (intracellular) fraction, and myelin-related and purified compact myelin fractions by immunoprecipitation with monospecific anti-apo A-I and or anti-Po antisera. Our results demonstrated that secreted apo A-I is fatty acylated, but not phosphorylated or sulfated. Avian Po protein was phosphorylated by a phorbol ester sensitive protein kinase. Sulfation, as well as fatty acylation, of avian Po protein was observed in organ culture using highly sensitive methods of detection. These results indicate that fatty acylation of secreted apo A-I and phosphorylation, sulfation and fatty acylation of Po have been conserved during evolution, and that these post-translational modifications may play ...Continue Reading

References

Mar 1, 1979·Journal of Neurochemistry·K UyemuraS Uehara
Oct 1, 1979·Canadian Journal of Physiology and Pharmacology·E H Petrali, P V Sulakhe
May 5, 1975·Biochimica Et Biophysica Acta·J M MatthieuR H Quarles
Mar 1, 1990·Journal of Neuroscience Research·L H van den BergN Latov
Dec 1, 1990·Journal of Neurochemistry·M SuzukiK Uyemura
Feb 1, 1990·Journal of Neuroscience Research·A C LeBlanc, J F Poduslo
Apr 1, 1987·Brain Research·A C LeBlancC Mezei
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A C LeBlanc, C Mezei
Dec 1, 1987·Journal of Neurochemistry·K R Brunden, J F Poduslo
Jan 1, 1988·Progress in Neurobiology·J B Ulmer
Apr 1, 1989·Journal of Neurochemistry·A HarelM Schwartz
Sep 21, 1989·Biochimica Et Biophysica Acta·J L DixonD Banerjee
Sep 1, 1989·Journal of Neurochemistry·P J Gebicke-Haerter, E M Shooter
Sep 9, 1985·Biochimica Et Biophysica Acta·C L Ashendel
Jan 1, 1987·Progress in Brain Research·M J IgnatiusE M Shooter
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Mar 1, 1971·Canadian Journal of Biochemistry·Y W Hu, C Mezei
Sep 1, 1981·Journal of Neurochemistry·C Mezei, J A Verpoorte
Jan 1, 1984·Journal of Neurochemistry·D J Nunn, C Mezei
Sep 1, 1981·Neurochemical Research·K UyemuraK Kitamura
Mar 1, 1994·Journal of Neurochemistry·O A BizzozeroA Pastuszyn
Jun 12, 1993·Biochimica Et Biophysica Acta·S N MathurF J Field

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.