Abstract
Chondromodulin-I (ChM-I) is a small glycoprotein that is abundant in fetal cartilage. Mature chondromodulin-I is processed from a larger precursor form, presumably at a proteolytic site RERR-ELVR. The precursor, mature chondromodulin-I and two processed products, the remnant left after removal of mature chondromodulin-I and a smaller, unglycosylated form, were identified using antipeptide antisera. The products of chondromodulin-I precursor processing were seen in cultured chondrocytes, a stable long-term culture chondrosarcoma cell line, as well as Chinese hamster ovary (CHO) cells transfected with an expression plasmid that contained cDNA coding for the chondromodulin-I precursor. Pulse-chase analysis allowed a processing pathway to be analyzed for chondromodulin-I. To further dissect the processing events, three constructs that express recombinant wild-type or mutant chondromodulin-I were transfected into CHO cells. We showed that chondromodulin-I is cleaved intracellularly at the predicted cleavage site, and that the mature glycopeptide is rapidly secreted immediately after processing. The chondromodulin-1 precursor has a short half-life and is not readily apparent in tissue samples, suggesting that chondromodulin is not a ...Continue Reading
References
Mar 29, 1991·Biochemical and Biophysical Research Communications·Y HirakiF Suzuki
Nov 1, 1990·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A M KucharskaJ H Kimura
Feb 24, 1989·Cell·R BrachmannR Derynck
Sep 1, 1983·Infection and Immunity·J M Moehring, T J Moehring
Jan 1, 1993·Annual Review of Biochemistry·J Massagué, A Pandiella
Jan 24, 1998·The Journal of Biological Chemistry·Y HirakiJ Kondo
May 23, 1998·The Biochemical Journal·K Nakayama
Apr 2, 1999·European Journal of Biochemistry·Y HirakiJ Kondo
May 21, 1999·Biochemical and Biophysical Research Communications·F Suzuki
Aug 19, 1999·FEBS Letters·C ShukunamiY Hiraki
Sep 16, 1999·Cellular and Molecular Life Sciences : CMLS·P J NeameA Azizan
Oct 20, 1999·Frontiers in Bioscience : a Journal and Virtual Library·T M Hering
Dec 11, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·U H DietzR Balling
Mar 4, 2000·Brain Research·N G Seidah, M Chrétien
Jul 27, 2000·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Y Hiraki, C Shukunami
Nov 9, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·A AzizanP J Neame
Feb 13, 2001·Biochemical and Biophysical Research Communications·K YamanaM Kiyoki
Feb 13, 2001·Biochemical and Biophysical Research Communications·C ShukunamiY Hiraki
May 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O BrandauA Aszódi
Citations
Nov 19, 2004·Virchows Archiv : an International Journal of Pathology·Kimihide KusafukaSusumu Ikehara
Feb 2, 2008·Cell and Tissue Research·Chisa ShukunamiYuji Hiraki
Jun 14, 2005·Biochemical and Biophysical Research Communications·Chisa ShukunamiYuji Hiraki
Jan 15, 2004·Experimental Cell Research·Didier F PisaniClaude A Dechesne
Jul 13, 2002·Trends in Biochemical Sciences·Luis Sánchez-PulidoAlfonso Valencia
Sep 19, 2006·Nature Medicine·Masatoyo YoshiokaKeiichi Fukuda
Jun 28, 2002·Acta histochemica·Kimihide KusafukaTamiko Takemura
Jun 16, 2009·Molecular Pharmacology·Hongfei ZhouDavid Ross
Aug 23, 2002·Molecular and Cellular Biology·Oliver BrandauReinhard Fässler
Jan 6, 2005·Molecular and Cellular Biology·Denitsa DochevaOliver Brandau
Jul 16, 2005·Connective Tissue Research·Yuji Hiraki, Chisa Shukunami
Jun 23, 2009·Differentiation; Research in Biological Diversity·Stephane BoeufWiltrud Richter
Sep 17, 2008·Bone·Kiminori YukataNatsuo Yasui
Jun 15, 2011·The FEBS Journal·Hanna WillanderJenny Presto
Oct 9, 2013·Biochemistry·Stefan D KnightJan Johansson
Dec 25, 2007·The Journal of Biological Chemistry·Cordula Surmann-SchmittMichael Stock
Aug 15, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nandaraj TayeDirk Hubmacher
Mar 14, 2018·Genes·Myungchul SongChingtack Han
Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Sipin ZhuJiake Xu
Jun 20, 2017·Molecular Medicine Reports·Bing DengFuyou Wang
May 20, 2004·Journal of Cell Science·Yusuke OshimaChisa Shukunami