PMID: 6964399Oct 1, 1982Paper

Post-translational processing of cholecystokinin in pig brain and gut

Proceedings of the National Academy of Sciences of the United States of America
J EngR S Yalow

Abstract

A sequential extraction method employing methanol extraction of the COOH-terminal fragments of cholecystokinin (CCK) from pig tissues followed by HCl extraction of intact CCK and its NH2-terminal fragments is described. Radioimmunoassay of extracts and their fractionation by Sephadex chromatography and HPLC demonstrate that the distributions of COOH-terminal and NH2-terminal immunoreactivities among various regions of brain are similar and independent of the concentrations in individual regions. The distribution in gut differs from that in brain. Greatest concentrations of CCK immunoreactivity are located in cortical tissue in the brain and in duodenal mucosa in gut. Both brain and gut contain CCK octapeptide (CCK8) and an NH2-terminal fragment that is likely to be desoctapeptide-CCK33. Intact CCK33 is extractable from gut but not from brain. Brain contains another NH2-terminal immunoreactive molecule lacking COOH-terminal immunoreactivity that may be a peptide with a COOH-terminal extension, as has been described for gastrin, or one that may not be derived from a CCK33-like precursor. This peptide is much less prominent in gut, or may be nonexistent there. The failure to find CCK33 in the brain and the presence in the brain of...Continue Reading

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Citations

Jul 15, 1983·European Journal of Pharmacology·M FeketeG Telegdy
Feb 10, 1984·European Journal of Pharmacology·M FeketeG Telegdy
Nov 16, 1984·Journal of Immunological Methods·R F MurphyS N Joffe
Dec 30, 1985·Life Sciences·C BonatoR S Yalow
Sep 15, 1986·Life Sciences·C BonatoR S Yalow
Oct 1, 1984·Regulatory Peptides·V E EysseleinJ H Walsh
May 1, 1984·Peptides·K IchiharaR S Yalow
Nov 1, 1988·Peptides·C M TurkelsonJ R Reeve
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y ShinomuraR S Yalow
Jun 1, 1987·Brain Research Bulletin·Z W FanR S Yalow
Apr 12, 2000·Journal of Gastroenterology and Hepatology·M Otsuki
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J EngR S Yalow
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·U GublerJ Eng
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·V E EysseleinJ H Walsh
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J EngR S Yalow
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EngR S Yalow
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Feb 27, 1987·Biochemical and Biophysical Research Communications·Y ShinomuraR S Yalow
May 1, 1984·The Journal of Surgical Research·W T ChanceJ E Fischer
Oct 16, 1983·Brain Research·J L MeekO Giorgi
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Jan 1, 1985·Annals of the New York Academy of Sciences·J B Jansen, C B Lamers
Sep 1, 1991·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·T Takács, A Pap

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