Post-translational Regulation of Hexokinase Function and Protein Stability in the Aestivating Frog Xenopus laevis

The Protein Journal
Christine L Childers, K B Storey

Abstract

Xenopus laevis endure substantial dehydration which can impose hypoxic stress due to impaired blood flow. Tissues may increase reliance on anaerobic glycolysis for energy production making the regulation of hexokinase (HK) important. We investigated the enzymatic properties and phosphorylation state of purified HK from the muscle of control and dehydrated (30% total body water lost) frogs. Bioinformatic tools were also applied to analyze the structural implication of HK phosphorylation in silico. HK from the muscle of dehydrated frogs showed a significantly higher Vmax (3.4-fold) and Km for glucose (2.4-fold) compared with control HK but the Km for ATP was unaltered. HK from dehydrated frogs also showed greater phosphoserine content (20% increase) and lower phosphothreonine (22% decrease) content compared to control HK. Control HK had a higher melting temperature (Tm = 61.9 °C) than from dehydrated (Tm = 54.2 °C) frogs when thermostability was tested using differential scanning fluorimetry. In silico phosphorylation of a Xenopus HK caused alterations in active site binding, corroborating phosphorylation as the probable mechanism for kinetic regulation. Physiological consequences of dehydration-induced HK phosphorylation appear ...Continue Reading

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Citations

Jul 15, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Cheng-Wei WuKenneth B Storey
Nov 21, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Razvan C StanMaristela M de Camargo
Jan 12, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Christine L Childers, Kenneth B Storey
Feb 18, 2021·Molecular and Cellular Biochemistry·Bryan E LuuKenneth B Storey
Jun 28, 2020·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Michael B SmolinskiKenneth B Storey

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