Post-transplant recurrent hepatitis C: immunohistochemical detection of hepatitis C virus core antigen and possible pathogenic implications

Liver International : Official Journal of the International Association for the Study of the Liver
Mário G PessôaTeresa L Wright

Abstract

The mechanisms by which severe cholestatic hepatitis develops after liver transplantation are not fully understood. Reports on immunohistochemical distribution of hepatitis C virus (HCV) antigens are still scarce, but recently, HCV immunostaining was suggested for early diagnosis of cholestatic forms of recurrent hepatitis C in liver grafts. After purification, Rb246 pab anticore (aa1-68) yielded specific, granular cytoplasmic staining in hepatocytes. Signal amplification through the Envision-Alkaline Phosphatase System avoided endogenous biotin and peroxidase. Rb246 was applied to liver samples of explants of 12 transplant recipients, six with the most severe form of post-transplantation recurrence, severe cholestatic hepatitis (group 1) and six with mild recurrence (group 2). We also assessed immuno-reactivity at two time-points post-transplantation (median 4 and 22 months) in both groups. HCV-core Ag was semiquantified from 0 to 3+ in each time point. Serum HCV-RNA was also measured on the different time points by branched DNA. In the early post-transplant time point, one patient had a mild staining (1+), two patients had a moderate staining (2+) and the other three had no staining in group 1, compared with five patients wit...Continue Reading

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Citations

Mar 25, 2014·World Journal of Gastroenterology : WJG·Francesco VasuriAntonia D'Errico-Grigioni
Jul 28, 2009·Gastroenterology·Yuqiong LiangStanley M Lemon
Jan 1, 2014·Transplant Infectious Disease : an Official Journal of the Transplantation Society·J HowellP Gow
Apr 4, 2013·Journal of Hepatology·Laura MensaXavier Forns
Mar 18, 2009·Human Pathology·Jay H Lefkowitch
May 14, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S RenX Chen
May 7, 2010·Current Opinion in Organ Transplantation·Ayse AytamanNorah A Terrault

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