Post-weaning blood transcriptomic differences between Yorkshire pigs divergently selected for residual feed intake

BMC Genomics
Haibo LiuChristopher K Tuggle

Abstract

Improving feed efficiency (FE) of pigs by genetic selection is of economic and environmental significance. An increasingly accepted measure of feed efficiency is residual feed intake (RFI). Currently, the molecular mechanisms underlying RFI are largely unknown. Additionally, to incorporate RFI into animal breeding programs, feed intake must be recorded on individual pigs, which is costly and time-consuming. Thus, convenient and predictive biomarkers for RFI that can be measured at an early age are greatly desired. In this study, we aimed to explore whether differences exist in the global gene expression profiles of peripheral blood of 35 to 42 day-old pigs with extremely low (more efficient) and high RFI (less efficient) values from two lines that were divergently selected for RFI during the grow-finish phase, to use such information to explore the potential molecular basis of RFI differences, and to initiate development of predictive biomarkers for RFI. We identified 1972 differentially expressed genes (DEGs) (q ≤ 0.15) between the low (n = 15) and high (n = 16) RFI groups of animals by using RNA sequencing technology. We validated 24 of 37 selected DEGs by reverse transcription-quantitative PCR (RT-qPCR) in a joint analysis o...Continue Reading

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Citations

Jun 26, 2017·BMC Genomics·Haibo LiuChristopher K Tuggle
Apr 11, 2019·Frontiers in Genetics·Pâmela A AlexandreHeidge Fukumasu
May 24, 2017·Research in Veterinary Science·Timothy A GilesNeil Foster

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Methods Mentioned

BETA
biopsies
PCR
RNA-seq
chip
MDS
RNAseq

Software Mentioned

Fluidigm
rna
MDS
STAR
limma
topGO ”
R package “ QuasiSeq ”
Integrated Pathway Analysis Database ( IPAD )
featureCounts
IPAD

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