Jul 31, 2013

Posterior elongation in the annelid Platynereis dumerilii involves stem cells molecularly related to primordial germ cells

Developmental Biology
Eve GazaveMichel Vervoort

Abstract

Like most bilaterian animals, the annelid Platynereis dumerilii generates the majority of its body axis in an anterior to posterior temporal progression with new segments added sequentially. This process relies on a posterior subterminal proliferative body region, known as the "segment addition zone" (SAZ). We explored some of the molecular and cellular aspects of posterior elongation in Platynereis, in particular to test the hypothesis that the SAZ contains a specific set of stem cells dedicated to posterior elongation. We cloned and characterized the developmental expression patterns of orthologs of 17 genes known to be involved in the formation, behavior, or maintenance of stem cells in other metazoan models. These genes encode RNA-binding proteins (e.g., tudor, musashi, pumilio) or transcription factors (e.g., myc, id, runx) widely conserved in eumetazoans. Most of these genes are expressed both in the migrating primordial germ cells and in overlapping ring-like patterns in the SAZ, similar to some previously analyzed genes (piwi, vasa). The SAZ patterns are coincident with the expression of proliferation markers cyclin B and PCNA. EdU pulse and chase experiments suggest that new segments are produced through many rounds of...Continue Reading

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  • Citations19

Citations

Mentioned in this Paper

Vertebrates
Embryo
Body Regions
Congenital Kyphosis
Body Part
Anethum graveolens
Biochemical Pathway
Neural Tube
Jackdaws
Physiologic Pulse

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