Postimport methylation of the small subunit of ribulose-1,5-bisphosphate carboxylase in chloroplasts

FEBS Letters
R GrimmJ Soll

Abstract

Electron impact mass spectronomy analysis of the amino-terminal amino acid of the small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase (Rubisco) showed that the amino-terminal methionine residue is post-translationally modified to N-methyl-methionine. Modification of the amino-terminal methionine residue was found in mature SSU proteins from the dicotyledonous plants pea and spinach as well as the monocotyledonous plants barley and corn. SSU methyltransferase is a soluble protein in the chloroplast stroma and accepts heterologously expressed non-methylated SSU as a substrate using S-adenosylmethionine as methyl-group donor. We show that this modification occurs after post-translational uptake of the precursor form of SSU into chloroplasts and processing to its mature size. This reaction represents a new step in the import and assembly pathway of Rubisco holoenzyme.

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Citations

Jun 5, 2003·Archives of Biochemistry and Biophysics·Robert L Houtz, Archie R Portis
Oct 25, 2000·Trends in Plant Science·K Jan van Wijk
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Oct 20, 2001·The Journal of Biological Chemistry·T C TaylorI Andersson

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