PMID: 9435608Jan 22, 1998Paper

Postischemic endothelium-dependent vascular reactivity is preserved in adhesion molecule-deficient mice

The American Journal of Physiology
M A BandaD N Granger

Abstract

Previous studies utilizing monoclonal antibodies directed against specific leukocyte-endothelial cell adhesion proteins have suggested that neutrophils mediate endothelial cell injury in a number of vascular beds after ischemia-reperfusion (I/R). In the present study, we investigated superior mesenteric artery (SMA) vascular reactivity to acetylcholine (ACh) and sodium nitroprusside (SNP) after occlusion and reperfusion in wild-type (C57BL/6) mice and in gene-targeted mice that are deficient in either CD11/CD18, intercellular adhesion molecule 1 (ICAM-1), or P-selectin. All mice were 4 wk of age, and the SMA was occluded for 45 min and then reperfused for 45 min. Segments of SMA were isolated and suspended in organ chambers and contracted with phenylephrine (10(-5) M), and the maximal vasorelaxation to ACh (10(-6) M) and SNP (10(-6) M) was measured. SMA from sham-operated C57BL/6 mice relaxed 83.5 +/- 3.3% to ACh and 91.7 +/- 3.4% to SNP. In contrast, segments of SMA from C57BL/6 mice subjected to I/R demonstrated a marked impairment in vasorelaxation to ACh (51.3 +/- 4.7%, P < 0.01 vs. sham) without any impairment in the vasoreactivity to SNP (86.1 +/- 5.5%). In CD11/CD18-deficient mice, SMA reactivity to ACh (84.7 +/- 2.3%) a...Continue Reading

References

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P KubesD N Granger
Aug 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L EntmanC W Smith
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J L ZweierM L Weisfeldt
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J E SlighA L Beaudet

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Citations

Jul 10, 2002·ILAR Journal·Craig J HartleyLloyd H Michael
Mar 10, 2000·American Journal of Physiology. Heart and Circulatory Physiology·S Bertuglia, A Colantuoni
Aug 6, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mikiji MoriD Neil Granger

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