PMID: 8084042Sep 1, 1994Paper

Postischemic extremities exhibit immediate release of tumor necrosis factor

Journal of Vascular Surgery
W C SternberghA A Fowler

Abstract

Although reperfusion of acutely ischemic extremities can cause cardiopulmonary collapse and death, the humoral agent(s) released from limbs promoting this distant organ injury are not well characterized. We hypothesized that tumor necrosis factor-alpha (TNF-alpha), a cytokine that causes cardiopulmonary dysfunction in septic shock, may be released from postischemic extremities. Isolated rat hindlimbs were perfused at constant pressure with a nonrecirculating crystalloid-based buffer. After 60 or 120 minutes of normothermic ischemia, TNF activity was measured in sequential samples of venous effluent by L929 bioassay. Associated limb injury was assessed by the extent of no-reflow after reperfusion, changes in endothelial permeability to iodine 125-labeled albumin and skeletal muscle injury by uptake of technetium 99 pyrophosphate. After 120 minutes of normothermic ischemia (n = 10), a 15-fold increase in TNF-alpha activity in venous effluent occurred, with peak activity at 1.5 minutes of reperfusion (30.6 +/- 8.7 U/ml) falling to near control levels by 5 minutes. This group had a 3.3-fold increase in vascular permeability, a 2.2-fold increase in the muscle injury index and a 71.2% decline in reperfusion flow (all p < 0.05 vs cont...Continue Reading

Citations

Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zivana TezakLauren M Pachman
Oct 3, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Robert S CrawfordMichael T Watkins
Feb 11, 1998·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·G GermannH U Steinau
Apr 25, 2000·Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists·N M ElmarasyB R Hopkinson
Aug 14, 2010·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Terence J Coderre, Gary J Bennett
Feb 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·T D Warner, P Klemm
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May 25, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Dhiraj JoshiJanice C Tsui
Oct 27, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·T Clementsen, O Reikerås
Jan 10, 2018·Frontiers in Cellular Neuroscience·Luis F QuemeMichael P Jankowski
Dec 12, 2001·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·M J BownR D Sayers

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