Postischemic poly (ADP-ribose) polymerase (PARP) inhibition reduces ischemia reperfusion injury in a hind-limb ischemia model.

Surgery
Robert S CrawfordMichael T Watkins

Abstract

Several experiments were designed to determine whether the systemic, postischemic administration of PJ34,which is a poly-adenosine diphosphate (ADP)-ribose polymerase inhibitor, decreased tissue injury and inflammation after hind-limb ischemia reperfusion (I/R). C57BL6 mouse limbs were subjected to 1.5 h ischemia followed by 24-h reperfusion. The treatment group (PJ) received intraperitoneal PJ34 (30 mg/kg) immediately before reperfusion, as well as 15 min and 2 h into reperfusion. The control group (CG) received lactated Ringer's alone at the same time intervals as PJ34 administration. The skeletal muscle levels of adenosine triphosphate (ATP), macrophage inflammatory protein-2 (MIP-2), keratinocyte derived chemokine (KC), and myeloperoxidase (MPO) were measured. Quantitative measurement of skeletal muscle tissue injury was assessed by microscopic analysis of fiber injury. ATP levels were higher in limbs of PJ versus CG mice (absolute ATP: 4.7 +/- 0.35 vs 2.3 +/- 0.15-ng/mg tissue, P = .002). The levels of MIP-2, KC, and MPO were lower in PJ versus CG mice (MIP-2: 1.4 +/- 0.34 vs 3.67 +/- 0.67-pg/mg protein, P = .014; KC: 4.97 +/- 0.97 vs 12.65 +/- 3.05-pg/mg protein, P = .037; MPO: 46.27 +/- 10.53 vs 107.34 +/- 13.58-ng/mg pr...Continue Reading

References

Sep 1, 1994·Cardiovascular and Interventional Radiology·H J WagnerP Reuter
Oct 1, 1996·World Journal of Surgery·M M YassinB J Rowlands
Mar 21, 1998·The British Journal of Surgery·B D BraithwaiteJ J Earnshaw
Jul 22, 1998·The Journal of Thoracic and Cardiovascular Surgery·E M BoyleE D Verrier
Jul 27, 2001·The Journal of Surgical Research·A WakaiH P Redmond
Jan 5, 2002·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J G MableyC Szabó
Sep 12, 2002·Pharmacological Reviews·László Virág, Csaba Szabó
Jan 21, 2004·The Journal of Surgical Research·James A BonheurMichael T Watkins
Apr 30, 2004·The Journal of Thoracic and Cardiovascular Surgery·Y Joseph WooTodd Grand
Aug 17, 2005·Journal of Vascular Surgery·Hong T HuaMichael T Watkins
May 30, 2006·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Gábor SzabóSusanne Bährle
Sep 11, 2007·Apoptosis : an International Journal on Programmed Cell Death·Chantal EthierGuy G Poirier
May 24, 2008·The Journal of Surgical Research·Thomas A AbbruzzeseMichael T Watkins
Sep 10, 2009·Journal of the National Cancer Institute·Rabiya S Tuma

❮ Previous
Next ❯

Citations

Jun 11, 2014·Inflammation·Yilin ZhaoQi Luo
Jul 10, 2012·Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists·Kuei-Yao HsuChun-Jen Huang
Mar 25, 2014·The Journal of Surgical Research·Hassan AlbadawiMichael T Watkins
Mar 4, 2021·Cell Chemical Biology·Chiho KimYonghao Yu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.