Postmortem Vitreous Humor Analysis in the Dog, Cat, and Horse

Journal of Analytical Toxicology
Adam W Stern, Manavi Muralidhar

Abstract

Postmortem chemistry can be a useful ancillary technique that the forensic pathologist can use during a death investigation. In stark contrast, there is limited information available for use of postmortem vitreous humor analysis in animals. In order to use postmortem vitreous humor in veterinary forensic investigations, validation of a method to analyze vitreous humor is required. The goal of this study was to determine the precision, bias, TEobs, and sigma (σ) of the Element DC chemistry analyzer, assess its precision using vitreous humor collected postmortem from dogs, cats, and horses, and assess the stability of postmortem vitreous humor from all 3 species. Analysis of quality control material (QCM) and pooled vitreous humor samples for the three species was used to test for sodium (Na), chloride (Cl), potassium (K), magnesium (Mg), creatinine (Crea), and blood/vitreous urea nitrogen (BUN/VUN). Analysis of QCM showed that the Element DC was both precise and accurate. When analyzing the pooled vitreous humors, most within-run CVs were found to be <5% and the between-run CVs for 5 out of 6 analytes were all found to be <5% for the dog, cat, and horse. In all three species, capped samples of vitreous humor were stable out of t...Continue Reading

References

Apr 1, 1972·Forensic Science·G Adjutantis, A Coutselinis
Jul 1, 1965·Medicine, Science, and the Law·W M Hughes
Jan 1, 1996·Veterinary Clinical Pathology·Anne Lanevschi, John W. Kramer
Jun 20, 2006·Forensic Science International : Synergy·Burkhard Madea, Frank Musshoff
Jan 1, 2008·Forensic Science, Medicine, and Pathology·Angela ByramjiRoger W Byard
Sep 10, 2009·Journal of Forensic Sciences·Keith W ProctorJohn C New
Sep 29, 2011·International Journal of Legal Medicine·Cristian Palmiere, Patrice Mangin
Aug 13, 2013·Journal of Analytical Toxicology·UNKNOWN Scientific Working Group for Forensic Toxicology
Dec 11, 2013·Veterinary Clinical Pathology·Kendal E HarrUNKNOWN ASVCP
Oct 23, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Katie M BoesStephen R Werre

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