Postnatal development of the bronchiolar club cells of distal airways in the mouse lung: stereological and molecular biological studies

Cell and Tissue Research
Srikanth KarnatiEveline Baumgart-Vogt

Abstract

Club (Clara) cells are nonciliated secretory epithelial cells present in bronchioles of distal pulmonary airways. So far, no information is available on the postnatal differentiation of club cells by a combination of molecular biological, biochemical, and stereological approaches in the murine lung. Therefore, the present study was designed to investigate the changes in the club cell secretory proteins (CC10, surfactant proteins A, B and D) and club cell abundance within the epithelium of bronchioles of distal airways during the postnatal development of the mouse lung. Perfusion-fixed murine lungs of three developmental stages (newborn, 15-day-old and adult) were used. Frozen, unfixed lungs were used for cryosectioning and subsequent laser-assisted microdissection of bronchiolar epithelial cells and RT-PCR analyses. High resolution analyses of the three-dimensional structures and composition of lung airways were obtained by scanning electron microscopy. Finally, using design-based stereology, the total and average club cell volume and the volume of secretory granules were quantified by light and transmission electron microscopy. Our results reveal that murine club cells are immature at birth and differentiate postnatally. Furth...Continue Reading

References

Sep 28, 1978·Biochimica Et Biophysica Acta·W M ManiscalcoJ B Warshaw
Jun 8, 1978·The New England Journal of Medicine·M CosioP T Macklem
Dec 11, 1991·American Journal of Respiratory Cell and Molecular Biology·S HorowitzE R Cheng
Nov 1, 1990·American Journal of Respiratory Cell and Molecular Biology·R L AutenS Horowitz
Jul 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C D BedettiM L Wong-Chong
Sep 1, 1987·Journal of Microscopy·H J Gundersen, E B Jensen
Jan 1, 1972·Beiträge Zur Pathologie·R C Rosan, J M Lauweryns
Jan 1, 1970·Journal of Clinical Psychology·L Pfeifer
Jan 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Singh, S L Katyal
Sep 1, 1984·The American Journal of Physiology·G D MassaroD Massaro
Aug 1, 1981·The Journal of Cell Biology·J W Slot, H J Geuze
Aug 18, 1982·Biochimica Et Biophysica Acta·J R BourbonP M Farrell
Jan 1, 1995·The Journal of Allergy and Clinical Immunology·T Van VyveY Sibille
May 1, 1997·Toxicology and Applied Pharmacology·M V FanucchiC G Plopper
Aug 1, 1997·American Journal of Respiratory Cell and Molecular Biology·C J JohnstonB R Stripp
Oct 6, 1997·American Journal of Respiratory Cell and Molecular Biology·M V FanucchiC G Plopper
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·J SavovS L Young
Oct 6, 2001·American Journal of Respiratory Cell and Molecular Biology·T BetsuyakuR M Senior
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S Z WangK S Harrod
Nov 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·T M WatsonB R Stripp
Jan 12, 2002·The American Journal of Pathology·Gregory W LawsonCharles G Plopper
Aug 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Barry R StrippCharles G Plopper
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shan-Ze WangKevin S Harrod
Oct 1, 1962·Archives of Disease in Childhood·R E CUDMOREA MITHAL
Jan 5, 2005·Nature Reviews. Immunology·Jo Rae Wright
May 10, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Elina ToskalaCharles G Plopper
Jul 31, 2008·Histochemistry and Cell Biology·Srikanth Karnati, Eveline Baumgart-Vogt
Feb 21, 2009·Histochemistry and Cell Biology·Srikanth Karnati, Eveline Baumgart-Vogt
Sep 15, 2009·The International Journal of Biochemistry & Cell Biology·Susan D Reynolds, Alvin M Malkinson
Mar 13, 2010·The European Respiratory Journal·A Winkelmann, T Noack
Sep 21, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Katherine M SutherlandLaura S Van Winkle

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Citations

Feb 13, 2018·Oxidative Medicine and Cellular Longevity·Natalia El-MerhieSrikanth Karnati
May 23, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nicole P MalvinThaddeus S Stappenbeck
Sep 22, 2019·International Journal of Molecular Sciences·Chao-Yuan ChangChun-Jen Huang
Apr 23, 2021·Biochemical Pharmacology·Soumeya AbedAnthony F Rullo
Jun 7, 2021·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Christoph WatermannSrikanth Karnati

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