Postnatal expression of KLF12 in the inner medullary collecting ducts of kidney and its trans-activation of UT-A1 urea transporter promoter

Biochemical and Biophysical Research Communications
Shin SudaShinichi Uchida

Abstract

Maturation of the inner medulla of the kidney occurs after birth and is vital for mammals to acquire maximal urinary concentrating ability. During this process, expression of several kidney transporters and channels involved in urine concentrating mechanisms is known to be regulated. We previously isolated KLF15 as a transcription factor that regulates the expression of the ClC-K1 chloride channel. We have now found that another KLF transcription factor, KLF12, is expressed in the kidney from around 15 days after birth. To gain insight into its involvement in the maturation process of the inner medulla, we first determined the expression site of KLF12 within the kidney by in situ hybridization. By comparing the AQP2 immunolocalization in sequential sections, KLF12 was found to be expressed in the collecting ducts. Because expression of the urea transporter UT-A1 and amiloride-sensitive epithelial sodium channels ENaC is known to be tightly regulated in the collecting ducts after birth, we tested whether KLF12 has a regulatory role in the promoter activities of these genes. KLF12 is able to increase UT-A1 but not ENaC promoter activity through the binding to CACCC motif. These results suggest that KLF12 is involved in the matura...Continue Reading

References

Jan 1, 1995·The Journal of Clinical Investigation·S UchidaF Marumo
Jun 15, 1999·Trends in Biochemical Sciences·J Turner, M Crossley
Sep 21, 2000·The Journal of Biological Chemistry·Y NakayamaS M Bagnasco
Oct 29, 2000·American Journal of Physiology. Renal Physiology·S Uchida
Jan 3, 2001·American Journal of Physiology. Renal Physiology·N AkizukiF Marumo
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·D T DangV W Yang
Jul 10, 2001·The Journal of Biological Chemistry·J J Bieker
Feb 8, 2002·American Journal of Physiology. Renal Physiology·Wen LiuMasashi Imai
Feb 8, 2002·American Journal of Physiology. Renal Physiology·Young-Hee KimJin Kim
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Christie P ThomasOmar A Itani

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Citations

May 1, 2012·The Journal of Biological Chemistry·Ying ZhangGary S Stein
Oct 25, 2007·Physiological Genomics·Panapat UawithyaMark A Knepper
Oct 21, 2010·Physiological Reviews·Beth B McConnell, Vincent W Yang
Mar 20, 2013·Molecular Aspects of Medicine·Chairat ShayakulMatthias A Hediger
Mar 6, 2013·Biochemical and Biophysical Research Communications·Xiaoyue ShenHaixiang Sun
Jul 10, 2009·International Journal of Cancer. Journal International Du Cancer·Yu NakamuraTatsuhiro Shibata
Feb 18, 2015·The Journal of Clinical Investigation·Jeffrey B Kopp
Nov 18, 2016·American Journal of Physiology. Renal Physiology·Sandeep K MallipattuJohn C He
Feb 25, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yubin ShinJong Hoon Park
Mar 2, 2017·Development·Agnieszka B BialkowskaSandeep K Mallipattu
Jul 14, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Viola C LamLewis L Lanier
Nov 14, 2014·American Journal of Physiology. Renal Physiology·Hong WangXiaoming Zhou
May 28, 2019·Pflügers Archiv : European journal of physiology·Yiwei ZhangXiaowei Pan

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