Postnatal ontogeny of striatal-enriched protein tyrosine phosphatase (STEP) in rat striatum

Experimental Neurology
A OkamuraY Ushio

Abstract

The present study was undertaken to examine developmental change in expression of striatal-enriched protein tyrosine phosphatase (STEP) in the postnatal striatum of rats. For this purpose, immunohistochemical staining and transimmunoblotting analyses were carried out using a cDNA-generated polyclonal antibody to the STEP with a molecular weight of 46 kDa. Immunostaining showed that in neonatal striatum STEP-immunoreactivity was found in discrete patches composed of many immature cells, which corresponded to the tyrosine hydroxylase-immunopositive "dopamine islands." With development there was an increase in staining intensity and in the number of positively reacting cells. By 4 weeks postnatally, STEP-immunoreactivity was almost homogeneously distributed throughout the striatum, as was seen at the adult stage. Immunoblotting analysis showed that STEP protein expression abruptly increased from 2 to 4 weeks postnatally when it reached the adult level. These findings suggest that STEP is involved in development and maturation of the striatal neurons.

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Citations

May 18, 2016·Neural Plasticity·Marija KamcevaPaul J Lombroso
Aug 1, 2015·Molecular Neurobiology·Ana SaavedraEsther Pérez-Navarro
Sep 15, 2014·Neuroscience Research·Takatoshi Karasawa, Paul J Lombroso
Jul 2, 2021·Frontiers in Cell and Developmental Biology·Yacoubou Abdoul Razak MahamanFeiqi Zhu

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