PMID: 6169734Sep 1, 1981Paper

Postsynaptic density antigens: preparation and characterization of an antiserum against postsynaptic densities

The Journal of Cell Biology
M N SampedroC W Cotman

Abstract

Long-term immunization of rabbits with postsynaptic densities (PSD) from bovine brain produced an antiserum specific for PSD as judged by binding to subcellular fractions and immunohistochemical location at the light and electron microscope levels. (a) The major antigens of bovine PSD preparations were three polypeptides of molecular weight 95,000 (PSD-95), 82,000 (PSD-82), and 72,000 (PSD-72), respectively. Antigen PSD-95, also present in mouse and rat PSDs was virtually absent from cytoplasm, myelin, mitochondria, and microsomes from rodent or bovine brain. Antigens PSD-82 and PSD-72 were present in all subcellular fractions from bovine brain, especially in mitochondria, but were almost absent from rodent brain. The antiserum also contained low-affinity antibodies against tubulin. (b)Immunohistochemical studies were performed in mouse and rat brain, where antigen PSD-95 accounted for 90 percent of the antiserum binding after adsorption with purified brain tubulin. At the light microscope level, antibody binding was observed only in those regions of the brain where synapses are known to be present. No reaction was observed in myelinated tracts, in the neuronal cytoplasm, or in nonneuronal cells. Strong reactivity was observed ...Continue Reading

References

Dec 4, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·A I Matus, D H Taff-Jones
Jan 1, 1978·Annual Review of Neuroscience·V S Caviness, P Rakic
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F E BloomP Greengard
Oct 1, 1978·The Journal of Cell Biology·W S AdairU W Goodenough
Dec 13, 1977·Biochemistry·S A BerkowitzR C Williams
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Burridge
Sep 5, 1973·Nature: New Biology·A OroszG Adam
Nov 1, 1974·The Journal of Cell Biology·C W CotmanD Taylor
Nov 1, 1974·The Journal of Cell Biology·G BankerC W Cotman
Oct 1, 1974·Journal of Neurochemistry·O S Jorgensen, E Bock
Dec 1, 1972·The Journal of Cell Biology·C W Cotman, D Taylor
Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·P H Nafstad
Mar 1, 1980·The Journal of Cell Biology·S W Craig, C L Lancashire
Jan 1, 1963·Archives of Biochemistry and Biophysics·J T DODGED J HANAHAN
May 1, 1965·The Journal of Cell Biology·J H VENABLE, R COGGESHALL

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Citations

Sep 1, 1990·Brain Research. Brain Research Reviews·R K Calverley, D G Jones
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·P W Beesley
Sep 30, 1998·The European Journal of Neuroscience·M Carrión-VázquezM Nieto-Sampedro
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Nieto-SampedroC W Cotman
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M B KennedyN E Erondu
Sep 1, 1982·The Journal of Cell Biology·S D FlanaganG Crawford
Oct 1, 1983·The Journal of Cell Biology·D M Landis, T S Reese
Oct 30, 2013·The Journal of Experimental Medicine·Christine D PozniakJoseph W Lewcock
Aug 15, 2014·Molecular Biology of the Cell·Damien Garbett, Anthony Bretscher
Mar 24, 2007·Stem Cells·Stefan LiebauTobias M Boeckers
May 4, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gemma McGregorJenni Harvey
Mar 27, 2015·Molecular Pharmacology·Henry A Dunn, Stephen S G Ferguson
Dec 31, 2019·Neuroscience·Bruno José MoraesA Cristina Rego
May 30, 2021·Neuropharmacology·Vishaal RajaniMichael W Salter

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