Postsynaptic expression of an epidermal growth factor receptor regulates cholinergic synapse formation between identified molluscan neurons

The European Journal of Neuroscience
R E van KesterenN I Syed

Abstract

Epidermal growth factor (EGF) family members are conserved in both vertebrates and invertebrates. Recent studies suggest that EGF ligands in invertebrates may have neurotrophic actions that possibly compensate for the apparent absence of neurotrophins in these species. In this study, we have cloned an EGF receptor from the mollusk Lymnaea stagnalis (L-EGFR), and shown that L-EGFR is the receptor for a previously identified EGF-like peptide in Lymnaea, named Lymnaea EGF (L-EGF). Knock-down of L-EGFR expression prevented L-EGF-induced excitatory synapse formation between identified cholinergic neuron visceral dorsal 4 (VD4) and its postsynaptic partner left pedal dorsal 1 (LPeD1). Moreover, knock-down of L-EGFR also prevented synapse formation induced by Lymnaea brain conditioned medium, suggesting that L-EGF is the most important, if not the only, brain-derived factor that promotes excitatory cholinergic synapse formation in Lymnaea. Thus, our data establish canonical EGF/EGFR signaling as an important synaptotrophic mechanism in invertebrates.

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Citations

Apr 28, 2012·Journal of Molecular Histology·Nichole Schmold, Naweed I Syed
Mar 29, 2014·Frontiers in Synaptic Neuroscience·Aislinn J Williams, Hisashi Umemori
May 4, 2005·Molecular and Cellular Neurosciences·M T van DiepenR E van Kesteren
May 1, 2012·Journal of Bone and Mineral Metabolism·Hiroshi Kaji
Jun 1, 2010·The Journal of Experimental Biology·Colin D KennedyBarbara A Sorg

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