Postsynaptic protein mobility in dendritic spines: long-term regulation by synaptic NMDA receptor activation

Molecular and Cellular Neurosciences
Kamal SharmaAnn Marie Craig

Abstract

Reorganization of molecular components represents a cellular mechanism for synaptic plasticity. Dendritic spines, major sites for glutamatergic synapses, compartmentalize dynamic changes in molecular composition. Here, we use fluorescence recovery after photobleaching (FRAP) in cultured hippocampal neurons to show that spine proteins undergo continual exchange with extra-spine pools. Each spine component has a distinctive mobility: calcium/calmodulin activated protein kinase CaMKIIalpha > GluR1 AMPA glutamate receptor > PSD-95 scaffolding protein > NR1 NMDA glutamate receptor. Stimulation of synaptic NMDA receptors by a protocol that induces chemical LTP resulted in a long-lasting reduction in the mobility of spine CaMKIIalpha and an increased mobile fraction but slower kinetics for spine GluR1. Stimulation also increased the resistance of postsynaptic CaMKIIalpha to detergent extraction. These results suggest long-lasting changes in affinity of protein-protein interactions and/or ongoing alterations in exo/endocytosis. Such lasting changes in protein mobility may contribute to maintaining alterations in synaptic efficacy.

References

Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C C OuimetP Greengard
Aug 1, 1996·Current Opinion in Cell Biology·M D EhlersR L Huganir
Jun 1, 1997·Trends in Neurosciences·M B Kennedy
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J LlopisR Y Tsien
Jun 10, 1998·Neuron·M FischerA Matus
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·J Lippincott-SchwartzA Kenworthy
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·A DosemeciT S Reese
Dec 14, 2001·Biochemical and Biophysical Research Communications·K U Bayer, H Schulman
Apr 24, 2002·Neuron·Kenneth R Tovar, Gary L Westbrook
May 8, 2002·Nature Reviews. Neuroscience·J LismanH Cline
Aug 27, 2002·Nature Neuroscience·D ReiselJ N P Rawlins
Nov 14, 2002·Annual Review of Genetics·Anna MatyniaAlcino J Silva
Apr 3, 2003·Nature Reviews. Neuroscience·Daniel Choquet, Antoine Triller
Oct 15, 2003·Neuron·David S Bredt, Roger A Nicoll
Nov 19, 2003·Journal of Neurochemistry·Lucia VinadeAyse Dosemeci
Dec 5, 2003·The Biochemical Journal·Roger J Colbran
Jun 11, 2004·Nature·Masanori MatsuzakiHaruo Kasai
Sep 21, 2004·Nature Reviews. Neuroscience·Eunjoon Kim, Morgan Sheng
Oct 22, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nikolai OtmakhovJohn Lisman
Jul 29, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andy HudmonPaul De Koninck
Jul 21, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Toshihiko KuriuShigeo Okabe
Nov 8, 2011·Molecular and Cellular Endocrinology·Antoun El Chemaly, Nicolas Demaurex

❮ Previous
Next ❯

Citations

Aug 16, 2006·Cell and Tissue Research·Craig C GarnerNoam E Ziv
Apr 15, 2011·Journal of Computational Neuroscience·Shahid KhanThomas S Reese
Jan 6, 2012·Journal of Computational Neuroscience·Shahid KhanAyisha Shabbir
Dec 22, 2010·Bulletin of Mathematical Biology·Max R BennettWilliam G Gibson
Mar 1, 2012·Journal of Molecular Neuroscience : MN·Juan ChengChen Zhang
Jan 6, 2010·Molecular Neurobiology·Xiao-bin Wang, Qiang Zhou
Jan 21, 2009·Nature Reviews. Neuroscience·Wei Feng, Mingjie Zhang
Dec 21, 2010·Nature Reviews. Neuroscience·Roger L Redondo, Richard G M Morris
Aug 1, 2013·Nature Reviews. Neuroscience·Cyril Hanus, Erin M Schuman
Feb 22, 2012·Nature Structural & Molecular Biology·Huiming LiPatrick G Hogan
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Thomas A BlanpiedMichael D Ehlers
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yan-Ping ZhangThomas G Oertner
May 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael Z LinRoger Y Tsien
Nov 4, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emile G BruneauMohammed Akaaboune
Jul 1, 2008·Annual Review of Cell and Developmental Biology·Yishi Jin, Craig C Garner
Mar 28, 2008·Neural Development·Josh L MorganRachel O L Wong
Nov 9, 2006·PLoS Biology·Noah W GrayKarel Svoboda
Dec 7, 2007·PLoS Computational Biology·Michael Graupner, Nicolas Brunel
Aug 9, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Vedakumar TatavartyGina G Turrigiano
Oct 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·James F SturgillBernardo L Sabatini
Oct 12, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Guillermo A YudowskiMark von Zastrow
Jan 13, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Justin M Kerr, Thomas A Blanpied
Apr 10, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Clarissa L WaitesCraig C Garner
Apr 29, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaobing ChenThomas S Reese
Apr 2, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chan-Ying ZhengRobert J Wenthold
Nov 11, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kim DoreRoberto Malinow
Nov 24, 2012·Brain Research Bulletin·Katalin Czöndör, Olivier Thoumine
Aug 21, 2012·Molecular and Cellular Neurosciences·Kevin SheAnn Marie Craig
Sep 17, 2011·Molecular and Cellular Neurosciences·Harold D MacGillavryThomas A Blanpied
Aug 3, 2011·Seminars in Cell & Developmental Biology·Elek Molnár
Mar 31, 2009·Trends in Cell Biology·Thomas M Newpher, Michael D Ehlers
Oct 4, 2008·Current Opinion in Neurobiology·Marianne RennerAntoine Triller
Aug 12, 2008·Neuropharmacology·Fernanda LaezzaJames E Huettner
Apr 22, 2008·Neuroscience·S D SantosC B Duarte
May 6, 2008·Neuroscience·F Jaskolski, J M Henley
Feb 27, 2007·Molecular and Cellular Neurosciences·Shigeo Okabe
Jun 19, 2015·Scientific Reports·Amber Petersen, Nashaat Z Gerges

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Michael C AshbyJeremy M Henley
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Toshihiko KuriuShigeo Okabe
Proceedings of the National Academy of Sciences of the United States of America
Xiaobing ChenThomas S Reese
© 2021 Meta ULC. All rights reserved