Postsynaptic responses of horizontal cells in the tiger salamander retina are mediated by AMPA-preferring receptors

Brain Research
J H YangS M Wu

Abstract

The postsynaptic responses of sign-preserving second-order retinal neurons (horizontal cells (HCs) and off-bipolar cells) are mediated by CNQX-sensitive AMPA/KA glutamate receptors. In this study we used receptor-specific allosteric regulators of desensitization and selected antagonists to determine the glutamate receptor subtypes in tiger salamander horizontal cells. Two approaches were employed in this study. The first was to measure postsynaptic currents induced by exogenously applied glutamate under voltage clamp conditions in living retinal slices; and the second was to record voltage responses controlled by endogenous glutamate released from photoreceptors in whole retinas. Application of 100 microM cyclothiazide (a specific AMPA receptor desensitization blocker) enhanced the glutamate-induced current by about 5 fold. In contrast, 300 microgram ml-1 Co nA (a specific kainate receptor desensitization blocker), had no effect. GYKI 52466 (a specific AMPA receptor antagonist) at 30 microM almost completely suppressed the glutamate-induced inward current in HCs. Cyclothiazide at 100 microM depolarized the HC dark membrane potential by about 5 mV and reduced the amplitudes of the voltage responses to dim lights, but enhanced th...Continue Reading

References

Sep 1, 1975·The Journal of Physiology·A Lasansky, S Vallerga
Jan 22, 1992·Proceedings. Biological Sciences·R A Shiells, G Falk
Jan 1, 1965·Cold Spring Harbor Symposia on Quantitative Biology·T Tomita
Jan 1, 1997·Visual Neuroscience·S Eliasof, C E Jahr

❮ Previous
Next ❯

Citations

Oct 24, 2007·Molecular Neurobiology·Wallace B Thoreson
Apr 20, 2001·Neuroscience Research·H HirasawaM Yamada
Feb 25, 2003·Progress in Retinal and Eye Research·Gilad TwigIdo Perlman
May 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jonathan D LintonJames B Hurley
Mar 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Katalin RablWallace B Thoreson
Oct 4, 2008·The Journal of Physiology·Deborah Langrill BeaudoinJonathan B Demb
Apr 19, 2008·The Journal of Physiology·Ji-Jie PangSamuel M Wu
Aug 15, 2012·The Journal of Physiology·Duco EndemanMaarten Kamermans
Jun 13, 2008·The European Journal of Neuroscience·Lucia CadettiWallace B Thoreson
Nov 4, 2008·Vision Research·Jian Zhang, Samuel M Wu
Jul 12, 2001·The Journal of Comparative Neurology·S HaverkampH Wässle
Sep 3, 2002·Journal of Neurophysiology·Dao-Qi ZhangDouglas G McMahon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.