Posttranscriptional control of the Salmonella enterica flagellar hook protein FlgE.

Journal of Bacteriology
Hee Jung Lee, Kelly T Hughes

Abstract

Previous work suggested that the FlgE (flagellar hook subunit) protein in Salmonella enterica serovar Typhimurium was posttranscriptionally regulated in response to the stage of flagellar assembly. Specifically, the FlgE protein could be detected in flagellar mutants defective at the stages of assembly before or after rod assembly but not in rod assembly mutants, yet flgE mRNA levels were unaffected. To elucidate posttranscriptional mechanisms involved in the coupling of flgE gene expression to hook assembly, the RNA sequences at the 5' and 3' ends of the flgE-containing mRNA processed from the large flgBCDEFGHIJKL operon were determined by rapid amplification of cDNA ends, and secretion of the FlgE protein in different flagellar assembly mutant strains was analyzed. The sequences 5' and 3' of the flgE gene where RNA processing occurred was within 15 bases upstream of the flgD stop codon and at bases 145 to 147 downstream of the flgF start codon, respectively. The ribosome binding site of the flgD gene was found to be inhibitory to flgE translation in strains deleted for the upstream flgD gene, unless the region 15 bases upstream of the flgD stop codon was present. Secretion of FlgE into the periplasm was monitored using beta-l...Continue Reading

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Citations

Aug 8, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sheng-Nan JiangJung-Joon Min
May 17, 2008·Nature Reviews. Microbiology·Fabienne F V Chevance, Kelly T Hughes
May 8, 2008·Nature Reviews. Microbiology·Ken F Jarrell, Mark J McBride
Jun 26, 2012·Journal of Bacteriology·Colleen R CourtneyDaniel B Kearns
Mar 2, 2016·Avian Pathology : Journal of the W.V.P.A·Jacqueline Boldrin de PaivaWanderley Dias da Silveira
Nov 19, 2009·Molecular Microbiology·Marc Erhardt, Kelly T Hughes
Feb 6, 2010·Molecular Microbiology·Marc ErhardtKelly T Hughes
Nov 14, 2014·PLoS Genetics·Marc ErhardtKelly T Hughes
Jan 25, 2008·Nature·Koushik PaulKelly T Hughes
Apr 4, 2021·International Journal of Molecular Sciences·Mathilde BouteillerAnnabelle Merieau
Jun 30, 2021·Nature Communications·Svenja HüsingThibaud T Renault

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