PMID: 11911366Mar 26, 2002Paper

Posttranscriptional modifications in the A-loop of 23S rRNAs from selected archaea and eubacteria

RNA
M A HansenBirte Vester

Abstract

Posttranscriptional modifications were mapped in helices 90-92 of 23S rRNA from the following phylogenetically diverse organisms: Haloarcula marismortui, Sulfolobus acidocaldarius, Bacillus subtilis, and Bacillus stearothermophilus. Helix 92 is a component of the ribosomal A-site, which contacts the aminoacyl-tRNA during protein synthesis, implying that posttranscriptional modifications in helices 90-92 may be important for ribosome function. RNA fragments were isolated from 23S rRNA by site-directed RNase H digestion. A novel method of mapping modifications by analysis of short, nucleotide-specific, RNase digestion fragments with Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) was utilized. The MALDI-MS data were complemented by two primer extension techniques using reverse transcriptase. One technique utilizes decreasing concentrations of deoxynucleotide triphosphates to map 2'-O-ribose methylations. In the other, the rRNA is chemically modified, followed by mild alkaline hydrolysis to map pseudouridines (psis). A total of 10 posttranscriptionally methylated nucleotides and 6 psis were detected in the five organisms. Eight of the methylated nucleotides and one psi have not been reported previously. Th...Continue Reading

References

Sep 25, 1987·Nucleic Acids Research·B Lankat-ButtgereitG Krupp
Dec 21, 1981·Nucleic Acids Research·G M VeldmanJ P Ebel
Jan 1, 1995·Annual Review of Biochemistry·E S Maxwell, M J Fournier
Jul 28, 1995·The Journal of Biological Chemistry·J A KowalakJ A McCloskey
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M O'Connor, A E Dahlberg
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J OfengandB G Lane
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
May 30, 1997·Cell·C M Smith, J A Steitz
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M BocchettaA S Mankin
Sep 15, 1998·Journal of Molecular Biology·B VesterS Douthwaite
Nov 14, 1998·Journal of Molecular Biology·P OstergaardR A Garrett
Dec 10, 1998·Nucleic Acids Research·J RozenskiJ A McCloskey
Jan 5, 2000·Molecular Cell·D F Kim, R Green
Apr 25, 2000·Science·A D OmerP P Dennis
May 18, 2000·Biochemical and Biophysical Research Communications·T CaldasG Richarme
Jun 3, 2000·RNA·C Rodriguez-FonsecaR A Garrett
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·S C Blanchard, J D Puglisi
Apr 3, 2001·Science·M M YusupovH F Noller

❮ Previous
Next ❯

Citations

Aug 9, 2003·Nucleic Acids Research·Christian Toft MadsenStephen Douthwaite
Dec 6, 2002·Nucleic Acids Research·Jonas Mengel-Jørgensen, Finn Kirpekar
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Birgitta SchweickertUlf B Göbel
May 26, 2011·RNA·Carsten A RaabeTimofey S Rozhdestvensky
Feb 7, 2007·RNA·Dorota Piekna-PrzybylskaMaurille J Fournier
May 18, 2004·RNA·Thomas Emil AndersenFinn Kirpekar
Jul 11, 2014·Molecular Biology of the Cell·Joanna RorbachMichal Minczuk
Nov 23, 2013·Critical Reviews in Biochemistry and Molecular Biology·Kevin C Baldridge, Lydia M Contreras
Feb 22, 2012·Journal of Proteomics·Anders M B Giessing, Finn Kirpekar
Sep 13, 2008·Journal of Molecular Biology·Elzbieta PurtaStephen Douthwaite
Aug 25, 2015·BMC Genomics·Patrick P DennisLennart Randau
Apr 16, 2003·Molecular Microbiology·Arina D OmerPatrick P Dennis
May 9, 2006·Journal of Molecular Biology·Niels Møller Andersen, Stephen Douthwaite
Apr 14, 2005·Journal of Molecular Biology·Finn KirpekarBirte Vester
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Taiga AraiTsutomu Suzuki
Jun 28, 2018·RNA Biology·Finn KirpekarBirte Vester
Aug 16, 2002·The Journal of Biological Chemistry·Jutta HagerUrsula Jakob
May 30, 2006·The Journal of Biological Chemistry·Jonas Mengel-JørgensenFinn Kirpekar
Oct 1, 2020·Cells·Ivan LaptevPetr Sergiev
Nov 15, 2002·The Journal of Biological Chemistry·Wayne A Decatur, Maurille J Fournier
Mar 30, 2021·Frontiers in Microbiology·Ruth BreuerLennart Randau
Mar 15, 2005·Analytical Chemistry·Zhaojing Meng, Patrick A Limbach
May 15, 2021·Wiley Interdisciplinary Reviews. RNA·Anwesha SarkarThomas J Begley
Sep 1, 2008·EcoSal Plus·Katherine S Long, Birte Vester
Mar 25, 2008·Analytica Chimica Acta·Anita Durairaj, Patrick A Limbach

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.