Posttranscriptional regulation of glutamine synthetase in the filamentous Cyanobacterium Anabaena sp. PCC 7120: differential expression between vegetative cells and heterocysts.

Journal of Bacteriology
Carla V GalmozziM Isabel Muro-Pastor

Abstract

Genes homologous to those implicated in glutamine synthetase (GS) regulation by protein-protein interaction in the cyanobacterium Synechocystis sp. strain PCC 6803 are conserved in several cyanobacterial sequenced genomes. We investigated this GS regulatory mechanism in Anabaena sp. strain PCC 7120. In this strain the system operates with only one GS inactivation factor (inactivation factor 7A [IF7A]), encoded by open reading frame (ORF) asl2329 (gifA). Following addition of ammonium, expression of gifA is derepressed, leading to the synthesis of IF7A, and consequently, GS is inactivated. Upon ammonium removal, the GS activity returns to the initial level and IF7A becomes undetectable. The global nitrogen control protein NtcA binds to the gifA promoter. Constitutive high expression levels of gifA were found in an Anabaena ntcA mutant (CSE2), indicating a repressor role for NtcA. In vitro studies demonstrate that Anabaena GS is not inactivated by Synechocystis IFs (IF7 and IF17), indicating the specificity of the system. We constructed an Anabaena strain expressing a second inactivating factor, containing the amino-terminal part of IF17 from Synechocystis fused to IF7A. GS inactivation in this strain is more effective than that ...Continue Reading

References

Jan 1, 1988·Methods in Enzymology·J Elhai, C P Wolk
Jul 1, 1996·Journal of Bacteriology·M I Muro-PastorF J Florencio
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·M García-DomínguezF J Florencio
Jan 3, 2001·Journal of Bacteriology·A HerreroE Flores
Aug 2, 2001·The Journal of Biological Chemistry·M I Muro-PastorF J Florencio
Sep 7, 2005·Photosynthesis Research·M Isabel Muro-PastorFrancisco J Florencio
Feb 21, 2006·FEMS Microbiology Letters·Alicia María Muro-PastorEnrique Flores
Sep 6, 2006·Journal of Bacteriology·Elvira Olmedo-VerdAntonia Herrero
May 18, 2007·Annual Review of Microbiology·John A Leigh, Jeremy A Dodsworth

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Citations

Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jan MitschkeAlicia M Muro-Pastor
Aug 6, 2013·The Science of the Total Environment·M Angeles Muñoz-MartínFrancisca Fernández-Piñas
Mar 16, 2017·Protein Science : a Publication of the Protein Society·Concetta CozzaBruno Rizzuti
Aug 2, 2018·Environmental Microbiology·Antonia Herrero, Enrique Flores
May 6, 2019·Environmental Microbiology·Manuel Brenes-ÁlvarezAlicia M Muro-Pastor
Jun 7, 2019·The New Phytologist·Rocío Robles-RengelM Isabel Muro-Pastor
Oct 31, 2018·Life·Paul BolayStephan Klähn

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