Posttranscriptional Regulation of Interleukin-33 Expression by MicroRNA-200 in Bronchial Asthma

Molecular Therapy : the Journal of the American Society of Gene Therapy
Xin TangGuanghai Yang

Abstract

The importance of understanding how interleukin-33 (IL-33) is regulated (particularly by miRs) is critical in IL-33 biology, and evidence of this in asthma pathology is limited. MicroRNA profiling of cells isolated from bronchoalveolar lavage of 14 asthmatic patients and 11 healthy controls revealed miR-200b and miR-200c were significantly reduced in asthmatic patients compared with healthy controls. The reduction was validated in two independent models of allergen-induced allergic airway inflammation and further demonstrated to be inversely correlated with asthma severity, as well as increased IL-33 production in asthmatic patients. In addition, the miR-200b and miR-200c binding sites in the 3' UTR of IL-33 mRNA were identified by bioinformatics analysis and reporter gene assay. More importantly, introduction of miR-200b and miR-200c reduced, while inhibition of endogenous miR-200b and miR-200c increased, the induction of IL-33 expression in lung epithelial cells. Exogenous administration of miR-200b to lungs of mice with allergic inflammation resulted in a decrease in IL-33 levels and resolution of airway inflammation phenotype. In conclusion, miR-200b and miR-200c by regulating the expression of IL-33 have a role in bronchia...Continue Reading

Citations

Nov 20, 2019·Allergy, Asthma & Immunology Research·Styliani TakaNikolaos G Papadopoulos
Sep 21, 2018·Frontiers in Immunology·Hiroaki TakatoriHiroshi Nakajima
Dec 29, 2020·Therapeutic Advances in Respiratory Disease·Li XuYuan Zhang
Mar 6, 2021·Molecular Therapy. Nucleic Acids·Tadkamol KrongbarameeLiu Hong
Sep 23, 2021·Immunological Reviews·Kristina JohanssonKarl Mark Ansel

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