Posttranscriptional Regulation of RhBRC1 (Rosa hybrida BRANCHED1 ) in Response to Sugars is Mediated via its Own 3' Untranslated Region, with a Potential Role of RhPUF4 (Pumilio RNA-Binding Protein Family)

International Journal of Molecular Sciences
Ming WangSoulaiman Sakr

Abstract

The shoot branching pattern is a determining phenotypic trait throughout plant development. During shoot branching, BRANCHED1 (BRC1) plays a master regulator role in bud outgrowth, and its transcript levels are regulated by various exogenous and endogenous factors. RhBRC1 (the homologous gene of BRC1 in Rosa hybrida) is a main branching regulator whose posttranscriptional regulation in response to sugar was investigated through its 3'UTR. Transformed Rosa calluses containing a construction composed of the CaMV35S promoter, the green fluorescent protein (GFP) reporter gene, and the 3'UTR of RhBRC1 (P35S:GFP::3'UTR RhBRC1 ) were obtained and treated with various combinations of sugars and with sugar metabolism effectors. The results showed a major role of the 3'UTR of RhBRC1 in response to sugars, involving glycolysis/the tricarboxylic acid cycle (TCA) and the oxidative pentose phosphate pathway (OPPP). In Rosa vegetative buds, sequence analysis of the RhBRC1 3'UTR identified six binding motifs specific to the Pumilio/FBF RNA-binding protein family (PUF) and probably involved in posttranscriptional regulation. RhPUF4 was highly expressed in the buds of decapitated plants and in response to sugar availability in in-vitro-cultured ...Continue Reading

References

Jan 1, 1970·Naunyn-Schmiedebergs Archiv Für Pharmakologie·K Lange, E R Proft
Apr 3, 1997·Nature·J DoebleyL Hubbard
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M T Chan, S M Yu
Oct 21, 1998·The Plant Journal : for Cell and Molecular Biology·M T Chan, S M Yu
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Mar 27, 1999·Nature·R L WangJ Doebley
Jun 11, 1999·The Plant Journal : for Cell and Molecular Biology·P CubasE Coen
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·W H ChengP S Chourey
Aug 12, 2000·Journal of Experimental Botany·R A Richards
Feb 23, 2002·Trends in Genetics : TIG·Marvin WickensRoy Parker
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Shunichi Kosugi, Yuko Ohashi
Feb 13, 2003·The Plant Journal : for Cell and Molecular Biology·Taito TakedaChiharu Ueguchi
May 20, 2003·Current Opinion in Plant Biology·Nicholas J Kruger, Antje von Schaewen
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Jan 18, 2005·Current Opinion in Plant Biology·Susan I Gibson
May 13, 2006·Biochemical and Biophysical Research Communications·Mukesh JainJitendra P Khurana
Feb 20, 2007·The Plant Cell·José Antonio Aguilar-MartínezPilar Cubas
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
Jun 19, 2007·Nature Reviews. Genetics·Jack D Keene
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Feb 19, 2008·Plant Molecular Biology·Stefan SchwarzPeter Huijser
Nov 1, 2008·Plant Molecular Biology·Sae Shimizu-SatoHitoshi Mori
Mar 27, 2009·Plant Physiology·Philip B BrewerChristine A Beveridge
Apr 16, 2009·Biochemical and Biophysical Research Communications·Qing Liu, Yue-Qin Chen
May 20, 2009·Current Opinion in Cell Biology·Marina Chekulaeva, Witold Filipowicz
Aug 18, 2009·The FEBS Journal·Carlos W Francischini, Ronaldo B Quaggio
Oct 22, 2009·Plant, Cell & Environment·Tesfamichael H KebromScott A Finlayson
Jan 9, 2010·Current Opinion in Plant Biology·Sjef SmeekensFilip Rolland
Mar 5, 2010·Plant Signaling & Behavior·Tesfamichael H KebromScott A Finlayson
Apr 9, 2010·Plant, Cell & Environment·Tiffanie GiraultNathalie Leduc
Aug 6, 2010·PloS One·Yimo Liu, Diane C Bassham
Dec 16, 2010·The EMBO Journal·Claudia CosentinoVincenzo Costanzo
Feb 26, 2011·Plant Signaling & Behavior·Nazia AbbasiSang-Bong Choi
Jun 4, 2011·Plant, Cell & Environment·Clemence HenrySandrine Pelleschi-Travier
Oct 27, 2011·Gene·Martine A Collart, Olesya O Panasenko
Nov 2, 2011·Plant Physiology·Elizabeth A DunChristine A Beveridge

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Citations

Feb 6, 2021·Journal of Experimental Botany·Ming WangSoulaiman Sakr
Apr 28, 2021·Plant Science : an International Journal of Experimental Plant Biology·Andrey A KotovGeorgy A Romanov
Oct 31, 2021·The New Phytologist·Suyash B PatilXueyong Li

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Methods Mentioned

BETA
three-hybrid
transgenic
PCR

Software Mentioned

QMEAN
ImageJ
BLAST
MEGA7

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