PMID: 9171421Jun 1, 1997Paper

Posttranslational control of the algT (algU)-encoded sigma22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN)

Journal of Bacteriology
K MatheeDennis E Ohman

Abstract

Pseudomonas aeruginosa strains associated with cystic fibrosis are often mucoid due to the copious production of alginate, an exopolysaccharide and virulence factor. Alginate gene expression is transcriptionally controlled by a gene cluster at 68 min on the chromosome: algT (algU)-mucA-mucB (algN)-mucC (algM)-mucD (algY). The algT gene encodes a 22-kDa alternative sigma factor (sigma22) that autoregulates its own promoter (PalgT) as well as the promoters of algR, algB, and algD. The other genes in the algT cluster appear to regulate the expression or activity of sigma22. The goal of this study was to better understand the functional interactions between sigma22 and its antagonist regulators during alginate production. Nonmucoid strain PAO1 was made to overproduce alginate (indicating high algD promoter activity) through increasing sigma22 in the cell by introducing a plasmid clone containing algT from mucA22(Def) strain FRD1. However, the bacterial cells remained nonmucoid if the transcriptionally coupled mucB on the clone remained intact. This suggested that a stoichiometric relationship between sigma22 and MucB may be required to control sigma factor activity. When the transcription and translational initiation of algT were m...Continue Reading

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Citations

Feb 28, 2014·The Journal of Biological Chemistry·Kemin TanAndrzej Joachimiak
Mar 5, 2015·Pathogens·Louise Cullen, Siobhán McClean
May 31, 2019·Clinical Microbiology Reviews·Sankalp MalhotraDaniel J Wozniak
Aug 5, 2020·Journal of Bacteriology·Ashley R CrossJoanna B Goldberg
Jan 19, 1999·Annual Review of Microbiology·K T Hughes, K Mathee
Aug 10, 2005·Treatments in Respiratory Medicine·Hiroyuki Kobayashi
Nov 8, 2007·Molecular Microbiology·Cristina E Alvarez-MartinezSuely L Gomes
Jan 4, 2012·FEMS Microbiology Reviews·Ethan E Mann, Daniel J Wozniak
Jun 14, 2014·FEMS Microbiology Reviews·María A LlamasIain L Lamont
Dec 19, 2007·Molecular Microbiology·María A LlamasWilbert Bitter
Jun 18, 2010·Environmental Microbiology·Glenn F J DullaSteven E Lindow
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Apr 14, 2012·Molecular Microbiology·F Heath Damron, Joanna B Goldberg
Nov 13, 2012·Nucleic Acids Research·Deepak BalasubramanianKalai Mathee
Sep 14, 2010·Protein Science : a Publication of the Protein Society·Gu Chen, Xu Zhang
Apr 23, 2016·Environmental Microbiology·Jay T Lennon, Brent K Lehmkuhl
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Jun 22, 2016·Journal of Bacteriology·Eric MarkelBryan Swingle
Nov 20, 2019·Journal of Bacteriology·Zhongmeng BaoBryan Swingle
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Sep 4, 2014·Journal of Bacteriology·Olivier CailleKalai Mathee
Apr 30, 2013·Journal of Bacteriology·Ian A Macdonald, Meta J Kuehn

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