Posttranslational modification of brain tubulins from the Antarctic fish Notothenia coriiceps: reduced C-terminal glutamylation correlates with efficient microtubule assembly at low temperature

Biochemistry
V RedekerH W Detrich

Abstract

We have shown previously that the tubulins of Antarctic fish assemble into microtubules efficiently at low temperatures (-2 to +2 degrees C) due to adaptations intrinsic to the tubulin subunits. To determine whether changes in posttranslational glutamylation of the fish tubulins may contribute to cold adaptation of microtubule assembly, we have characterized C-terminal peptides from alpha- and beta-tubulin chains from brains of adult specimens of the Antarctic rockcod Notothenia coriiceps by MALDI-TOF mass spectrometry and by Edman degradation amino acid sequencing. Of the four fish beta-tubulin isotypes, nonglutamylated isoforms were more abundant than glutamylated isoforms. In addition, maximal glutamyl side-chain length was shorter than that observed for mammalian brain beta tubulins. For the nine fish alpha-tubulin isotypes, nonglutamylated isoforms were also generally more abundant than glutamylated isoforms. When glutamylated, however, the maximal side-chain lengths of the fish alpha tubulins were generally longer than those of adult rat brain alpha chains. Thus, Antarctic fish adult brain tubulins are glutamylated differently than mammalian brain tubulins, resulting in a more heterogeneous population of alpha isoforms an...Continue Reading

References

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Citations

Jan 22, 2008·Journal of the American Society for Mass Spectrometry·Matthew T OlsonAlfred L Yergey
May 15, 2012·Marine Genomics·Daniela GiordanoCinzia Verde
Aug 23, 2008·Gene·Lawrence J Tartaglia, Daniel H Shain
Aug 27, 2010·Journal of Fish Biology·D GiordanoC Verde
Oct 9, 2008·Analytical Biochemistry·Pascal Verdier-PinardDiane Braguer
Aug 11, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·John H PostlethwaitH William Detrich
Feb 3, 2006·Journal of Muscle Research and Cell Motility·Stefan Lakämper, Edgar Meyhöfer

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