Posttranslational modification of the NH2-terminal region of CXCL5 by proteases or peptidylarginine Deiminases (PAD) differently affects its biological activity.

The Journal of Biological Chemistry
Anneleen MortierPaul Proost

Abstract

Posttranslational modifications, e.g. proteolysis, glycosylation, and citrullination regulate chemokine function, affecting leukocyte migration during inflammatory responses. Here, modification of CXCL5/epithelial cell-derived neutrophil-activating protein-78 (ENA-78) by proteases or peptidylarginine deiminases (PAD) was evaluated. Slow CXCL5(1-78) processing by the myeloid cell marker aminopeptidase N/CD13 into CXCL5(2-78) hardly affected its in vitro activity, but slowed down the activation of CXCL5 by the neutrophil protease cathepsin G. PAD, an enzyme with a potentially important function in autoimmune diseases, site-specifically deiminated Arg(9) in CXCL5 to citrulline, generating [Cit(9)]CXCL5(1-78). Compared with CXCL5(1-78), [Cit(9)]CXCL5(1-78) less efficiently induced intracellular calcium signaling, phosphorylation of extracellular signal-regulated kinase, internalization of CXCR2, and in vitro neutrophil chemotaxis. In contrast, conversion of CXCL5 into the previously reported natural isoform CXCL5(8-78) provided at least 3-fold enhanced biological activity in these tests. Citrullination, but not NH(2)-terminal truncation, reduced the capacity of CXCL5 to up-regulate the expression of the integrin α-chain CD11b on ne...Continue Reading

References

Aug 15, 1991·Biochemical and Biophysical Research Communications·Y JiangD T Graves
Apr 1, 1989·The Journal of Clinical Investigation·A T LookS C Peiper
Dec 1, 1987·The Journal of Experimental Medicine·D F BaintonT A Springer
Apr 21, 1995·The Journal of Biological Chemistry·A Ben-BaruchD J Kelvin
May 1, 1995·The American Journal of Physiology·N W LukacsR M Strieter
Sep 1, 1994·The Journal of Clinical Investigation·A E KochR M Strieter
Aug 1, 1997·American Journal of Respiratory Cell and Molecular Biology·T ImaizumiG A Zimmerman
Nov 19, 1997·Journal of Leukocyte Biology·J Van DammeG Opdenakker
Mar 31, 1999·Immunology Today·D RiemannJ Langner
Aug 17, 1999·The Journal of Clinical Investigation·P MentenJ Van Damme
Jan 4, 2001·Immunological Reviews·P ScapiniM A Cassatella
Oct 28, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Erik R VossenaarGer J M Pruijn
Mar 23, 2004·Annual Review of Immunology·Antal Rot, Ulrich H von Andrian
Jun 15, 2005·Annual Review of Biochemistry·T M HandelA E Proudfoot
Jul 19, 2005·Cytokine & Growth Factor Reviews·Mieke GouwyJo Van Damme
Feb 25, 2006·Nature Reviews. Immunology·Carl Nathan
May 30, 2006·The International Journal of Biochemistry & Cell Biology·Bence GyörgyEdit I Buzás
Jun 27, 2006·Nature Reviews. Immunology·Christine T N Pham
Jul 19, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tamara LoosPaul Proost
Sep 5, 2006·Nature Clinical Practice. Rheumatology·Jo L EylesIan P Wicks
Nov 25, 2006·Hematology·Peter E Newburger
Nov 25, 2006·Nature Reviews. Immunology·Alberto MantovaniMassimo Locati
Feb 3, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Charles N SerhanJohn L Wallace
Apr 6, 2007·Journal of Leukocyte Biology·Thomas A HamiltonXiaoxia Li
May 5, 2007·Thrombosis and Haemostasis·Ian G ColditzAntal Rot
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria Gómez-GaviroFederico Díaz-González
Sep 14, 2007·Immunological Reviews·William M Nauseef
Jan 9, 2008·The International Journal of Biochemistry & Cell Biology·Christine T N Pham
May 6, 2008·Methods in Molecular Biology·Mary C Dinauer
Aug 1, 2008·Arthritis and Rheumatism·Andrew KinlochPatrick J Venables
Sep 17, 2008·Pharmacology & Therapeutics·Anneleen MortierPaul Proost

❮ Previous
Next ❯

Citations

Dec 24, 2011·PloS One·Eva A V MoelantsPaul Proost
Jul 10, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Adam RainczukAndrew N Stephens
Jul 12, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·John B ZamjahnGregory J Bagby
Dec 14, 2012·Annals of the Rheumatic Diseases·Olga Sanchez-PernauteAstrid Juengel
May 1, 2013·Immunology and Cell Biology·Eva A V MoelantsPaul Proost
Oct 19, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Kalle H SipiläJyrki Heino
Jan 17, 2013·Rapid Communications in Mass Spectrometry : RCM·Jin Young KimSeung-won Lee
Dec 15, 2010·Experimental Cell Research·Anneleen MortierPaul Proost
Feb 9, 2017·International Journal of Molecular Sciences·Martin J StoneJulie Sanchez
Feb 7, 2018·International Journal of Molecular Sciences·Daiane BoffPaul Proost
Oct 31, 2017·Cellular & Molecular Immunology·Rik JanssensPaul Proost
Apr 2, 2014·Infection and Immunity·Eva A V MoelantsPaul Proost
Apr 10, 2020·Journal of Leukocyte Biology·Mieke MetzemaekersPaul Proost
Nov 29, 2016·Frontiers in Immunology·Mieke MetzemaekersPaul Proost
Nov 30, 2018·International Journal of Molecular Sciences·Alessandro VacchiniElena Monica Borroni
Apr 4, 2021·International Journal of Molecular Sciences·Vincent MariauleMoez Rhimi
May 27, 2021·International Reviews of Immunology·Abraham U Morales-PrimoJaime Zamora-Chimal
Jul 3, 2021·International Journal of Molecular Sciences·Héla MkaouarMoez Rhimi
Jul 3, 2021·International Journal of Molecular Sciences·Maaike CockxSofie Struyf

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.