Posttranslational modifications of human inter-alpha-inhibitor: identification of glycans and disulfide bridges in heavy chains 1 and 2

Biochemistry
E H OlsenJan J Enghild

Abstract

Inter-alpha-inhibitor (IalphaI) is a serine proteinase inhibitor found in high concentrations in human plasma. The protein is composed of a light inhibitory chain called bikunin and two heavy chains of unknown function. The three polypeptide chains are covalently assembled via a carbohydrate cross-link [Enghild, J. J., Salvesen, G., Hefta, S. A., Thogersen, I. B., Rutherfurd, S., & Pizzo, S. V. (1991) J. Biol. Chem. 266, 747-751]. The aim of this study was to complete the primary structure by characterizing additional covalent posttranslational modifications of the heavy chains. Analysis revealed three N-linked oligosaccharides located on Asn251 and Asn554 of heavy chain 1 and on Asn64 of heavy chain 2: all these were complex biantennary structures composed of (Asn)-GlcNAc2-Man-(Man-GlcNAc-Gal-SA)2. In addition, the IalphaI heavy chains carried several O-linked glycans located on Thr619 of heavy chain 1 and a cluster of four O-linked oligosaccharides on Thr612, Ser619, Thr621, and Thr637 of heavy chain 2. The oligosaccharides were short (Ser/Thr)-GalNAc-Gal-SA trisaccharides. The IalphaI heavy chains contain nine Cys residues, of which eight are involved in disulfide bridges. The unpaired Cys residue residing on heavy chain 1, ...Continue Reading

References

Jan 15, 1989·European Journal of Biochemistry·M Diarra-MehrpourJ P Martin
May 15, 1989·European Journal of Biochemistry·W GebhardE Wachter
Jan 1, 1988·Annual Review of Biochemistry·T W RademacherR A Dwek
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Nov 3, 1971·Nature: New Biology·J P Segrest, L W Cunningham
Aug 12, 1969·Biochimica Et Biophysica Acta·R Chandrasekaran, R Balasubramanian
Aug 1, 1980·The Biochemical Journal·C A Sayers, A J Barrett
Jan 1, 1982·Annual Review of Biochemistry·G Ashwell, J Harford
Sep 15, 1981·Journal of Molecular Biology·J M Thornton
Nov 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G OpdenakkerR A Dwek

❮ Previous
Next ❯

Citations

May 23, 2001·Archives of Biochemistry and Biophysics·C FalkenbergB Akerström
Jul 31, 2002·Biochimica Et Biophysica Acta·Ida B ThøgersenJan J Enghild
Dec 12, 2013·Journal of the American Chemical Society·Huan WangWilfred A van der Donk
Aug 14, 2009·Molecular & Cellular Proteomics : MCP·Zsuzsanna Darula, Katalin F Medzihradszky
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Steen V PetersenJan J Enghild
Sep 14, 2011·Journal of the American Chemical Society·Huan Wang, Wilfred A van der Donk
May 3, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Steen V Petersen, Jan J Enghild
Nov 26, 2015·Molecular & Cellular Proteomics : MCP·Marcus HoffmannErdmann Rapp
May 21, 2004·The Journal of Biological Chemistry·Lisheng ZhuoKoji Kimata
Dec 29, 1998·The Journal of Biological Chemistry·A M BlomE Fries

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.